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Virginia Commonwealth/8 July 2009
From 2009.igem.org
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| + | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=05}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=06}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=07}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=08}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=09}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=10}} | ||
| + | |} | ||
| + | ==Wednesday 8 July 2009== | ||
===Results=== | ===Results=== | ||
''Afton and Maria'' | ''Afton and Maria'' | ||
| - | *Digestion was successful and DNA from both plasmids appeared on the gel after the electrophoresis test | + | *Digestion was successful and DNA from both plasmids (pSB4C5,pSB1C3) appeared on the gel after the electrophoresis test |
**Possibly pSB3K3 in the iGem stock is a bad part | **Possibly pSB3K3 in the iGem stock is a bad part | ||
| + | * Gel Electrophoresis Result | ||
| + | |||
| + | [[Image: 2009-07-08_digest04(psb1c3_and_psb4C5).jpg|350px|none|thumb|from left to right: pSB1C3,1kB ladder,pSB4C5]] | ||
| + | * Miniprep Results: | ||
| + | {| cellpadding="5" border="1" padding="5" | ||
| + | |+ | ||
| + | |- | ||
| + | ! Part No.!! A260 (nm) !! A280 (nm) !! A260/A280 !! Vol. (µL) !! Conc. (µg/mL) !! Amt (µg) | ||
| + | |- | ||
| + | | pSB4C5 || 0.051 || 0.032 || 1.594 || 102 || 47 || 4.79 | ||
| + | |- | ||
| + | | pSB1C3 || 0.049 || 0.029 || 1.690 || 98 || 34 || 3.33 | ||
| + | |} | ||
| + | |||
[[User:Trentay|Trentay]] 18:50, 8 July 2009 (UTC) | [[User:Trentay|Trentay]] 18:50, 8 July 2009 (UTC) | ||
---- | ---- | ||
| Line 11: | Line 63: | ||
* Digestion of backbones: pSB1C3, pSB4C5 | * Digestion of backbones: pSB1C3, pSB4C5 | ||
* Spectrometry of backbone miniprepped stocks | * Spectrometry of backbone miniprepped stocks | ||
| + | * Transform promoter 106, RET mCherry when cuvettes arrive | ||
[[User:Trentay|Trentay]] 16:49, 8 July 2009 (UTC) | [[User:Trentay|Trentay]] 16:49, 8 July 2009 (UTC) | ||
---- | ---- | ||
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* Digestion | * Digestion | ||
* Agarose Gel preparation/Electrophoresis test | * Agarose Gel preparation/Electrophoresis test | ||
| - | [[User:Trentay|Trentay]] | + | * Parts (J06702,J23106) were taken from iGem wells, electrotransformed, and plated. |
| + | [[User:Trentay|Trentay]] 21:26, 8 July 2009 (UTC) | ||
Latest revision as of 16:59, 13 August 2009
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Contents |
Wednesday 8 July 2009
Results
Afton and Maria
- Digestion was successful and DNA from both plasmids (pSB4C5,pSB1C3) appeared on the gel after the electrophoresis test
- Possibly pSB3K3 in the iGem stock is a bad part
- Gel Electrophoresis Result
.jpg)
- Miniprep Results:
| Part No. | A260 (nm) | A280 (nm) | A260/A280 | Vol. (µL) | Conc. (µg/mL) | Amt (µg) |
|---|---|---|---|---|---|---|
| pSB4C5 | 0.051 | 0.032 | 1.594 | 102 | 47 | 4.79 |
| pSB1C3 | 0.049 | 0.029 | 1.690 | 98 | 34 | 3.33 |
Trentay 18:50, 8 July 2009 (UTC)
Tasks
Afton and Maria
- Digestion of backbones: pSB1C3, pSB4C5
- Spectrometry of backbone miniprepped stocks
- Transform promoter 106, RET mCherry when cuvettes arrive
Trentay 16:49, 8 July 2009 (UTC)
Wetlab
Afton and Maria
- Digestion
- Agarose Gel preparation/Electrophoresis test
- Parts (J06702,J23106) were taken from iGem wells, electrotransformed, and plated.
Trentay 21:26, 8 July 2009 (UTC)
