Team:Newcastle/Project/Labwork/OurProtocols/Making Agar Plates

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=Preparing the agar powder=
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=Preparing the LB+agar powder=
Mix the contents below to prepare the agar powder required to make 10L of agar solution
Mix the contents below to prepare the agar powder required to make 10L of agar solution
*100gr tryptoptan
*100gr tryptoptan
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Mix the content very well before using the mixed powder
Mix the content very well before using the mixed powder
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=Preparing the agar solution=
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To prepare 1L of LB+agar solution
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*Put 500 ml of distilled water to a tube
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*Put 400ml of distilled water to a tube and 35 gr of LB+agar powder. Top the tube up to 500ml with distilled water
 +
*Add 10ul of antifoam to LB+agar tube
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*Put the tubes for autoclaving for half an hour
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*Put the tube with LB+agar into 55C-60C water bath.
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*Put the water tube into the sink to cool it down.
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*If necessary, add the antibiotics to LB+agar tube after the tube cools down.
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*Mix both tubes throughly
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*Pour the plates using roughly 25ml of the solution for each Petri dish using the aseptic technique
 +
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Revision as of 12:27, 14 August 2009

Preparing the LB+agar powder

Mix the contents below to prepare the agar powder required to make 10L of agar solution

  • 100gr tryptoptan
  • 50gr yeast extract
  • 50gr NaCl
  • 150 gr agar

Mix the content very well before using the mixed powder

Preparing the agar solution

To prepare 1L of LB+agar solution

  • Put 500 ml of distilled water to a tube
  • Put 400ml of distilled water to a tube and 35 gr of LB+agar powder. Top the tube up to 500ml with distilled water
  • Add 10ul of antifoam to LB+agar tube
  • Put the tubes for autoclaving for half an hour
  • Put the tube with LB+agar into 55C-60C water bath.
  • Put the water tube into the sink to cool it down.
  • If necessary, add the antibiotics to LB+agar tube after the tube cools down.
  • Mix both tubes throughly
  • Pour the plates using roughly 25ml of the solution for each Petri dish using the aseptic technique




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