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Team:UNIPV-Pavia/Notebook/Week2Aug
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== <html><font class="dayw_style">August, 10th</font></html> == | == <html><font class="dayw_style">August, 10th</font></html> == | ||
| + | |||
| + | *Digestion for: | ||
| + | {|cellpadding="20" | ||
| + | |B1-13(E-S)(X2) | ||
| + | |B2-5(E-S)(X2) | ||
| + | |B3-5(E-X)(X2) | ||
| + | |- | ||
| + | |B4-2(E-X)(X2) | ||
| + | |} | ||
| + | |||
| + | *Gel run for all of them (only 1 ul for B1-13(E-S)(X2) in order to check the length) | ||
| + | |||
| + | *Band purification for: | ||
| + | {|cellpadding="20" | ||
| + | |B2-5(E-S)(X2) | ||
| + | |B3-5(E-X)(X2) | ||
| + | |B4-2(E-X)(X2) | ||
| + | |} | ||
| + | |||
| + | *Ethanol precipitation with sodium acetate for B1-13(E-S)(X2). | ||
| + | |||
| + | *Ligation: | ||
| + | **B5 = B1(E-S) + B4(E-X) in pSB1AK3 | ||
| + | **B6 = B2(E-S) + B3(E-X) in pSB1AK3 | ||
| + | |||
| + | *We incubated the ligation reactions at 16°C overnight. | ||
<div align="right"> | <div align="right"> | ||
Revision as of 10:19, 15 August 2009
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Week from August 10th, to August 16th, 2009
Previous Week
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Next Week
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August, 10th
- Digestion for:
| B1-13(E-S)(X2) | B2-5(E-S)(X2) | B3-5(E-X)(X2) |
| B4-2(E-X)(X2) |
- Gel run for all of them (only 1 ul for B1-13(E-S)(X2) in order to check the length)
- Band purification for:
| B2-5(E-S)(X2) | B3-5(E-X)(X2) | B4-2(E-X)(X2) |
- Ethanol precipitation with sodium acetate for B1-13(E-S)(X2).
- Ligation:
- B5 = B1(E-S) + B4(E-X) in pSB1AK3
- B6 = B2(E-S) + B3(E-X) in pSB1AK3
- We incubated the ligation reactions at 16°C overnight.
August, 11st
August, 12nd
August, 13rd
August, 14th
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Previous Week
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Next Week
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