Team:Newcastle/Labwork/13 August 2009

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=Lab Session 13/08/09=
=Lab Session 13/08/09=
==<u>Sporulation Tuning/Chassis Team</u>==
==<u>Sporulation Tuning/Chassis Team</u>==
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===Summary===
[https://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/12_August_2009&action=edit&section=7 Yesterday's] germination attempt seems to have worked, but results are not yet clear. This may have been due to a calculation error for the lysozyme, where instead of adding 40ul of stock lysozyme per ml of buffer solution, 4ul of stock lysozyme was added instead.  
[https://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/12_August_2009&action=edit&section=7 Yesterday's] germination attempt seems to have worked, but results are not yet clear. This may have been due to a calculation error for the lysozyme, where instead of adding 40ul of stock lysozyme per ml of buffer solution, 4ul of stock lysozyme was added instead.  
Therefore, on [https://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/17_August_2009&action=edit&section=1 Monday], we intend to redo the experiment. See [https://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/17_August_2009&action=edit&section=1 Monday, 17th August] for more details.
Therefore, on [https://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/17_August_2009&action=edit&section=1 Monday], we intend to redo the experiment. See [https://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/17_August_2009&action=edit&section=1 Monday, 17th August] for more details.
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Today we are trying to transform ''Bacillus subtilis'' with gfp-rrnb integration vector using the [https://2009.igem.org/Team:Newcastle/Project/Labwork/OurProtocols/TransformBac Transformation Protocol]. Metal sensor team kindly inoculated ''B. subtilis'' cells into flask tubes and placed them into the shaking incubator. The overnight cultures were labelled 1, 2 and 3, while the control was labelled as 4.
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Today we are trying to transform ''Bacillus subtilis'' with gfp-rrnb integration vector using the [https://2009.igem.org/Team:Newcastle/Project/Labwork/OurProtocols/TransformBac Transformation Protocol]. Metal sensor team kindly inoculated ''B. subtilis'' cells into flask tubes and placed them into the shaking incubator. The overnight cultures were labelled 1, 2 and 3, while the control was labelled as 4. Our team used overnight culture tube 3 and control tube 4.
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===Results===
{{:Team:Newcastle/Footer}}
{{:Team:Newcastle/Footer}}
{{:Team:Newcastle/Right}}
{{:Team:Newcastle/Right}}

Revision as of 01:51, 16 August 2009

Contents

Lab Session 13/08/09

Sporulation Tuning/Chassis Team

Summary

Yesterday's germination attempt seems to have worked, but results are not yet clear. This may have been due to a calculation error for the lysozyme, where instead of adding 40ul of stock lysozyme per ml of buffer solution, 4ul of stock lysozyme was added instead.

Therefore, on Monday, we intend to redo the experiment. See Monday, 17th August for more details.

Today we are trying to transform Bacillus subtilis with gfp-rrnb integration vector using the Transformation Protocol. Metal sensor team kindly inoculated B. subtilis cells into flask tubes and placed them into the shaking incubator. The overnight cultures were labelled 1, 2 and 3, while the control was labelled as 4. Our team used overnight culture tube 3 and control tube 4.

Results



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