Team:Newcastle/Labwork/13 August 2009

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Today we are trying to transform ''Bacillus subtilis'' with gfp-rrnb integration vector using the [https://2009.igem.org/Team:Newcastle/Project/Labwork/OurProtocols/TransformBac Transformation Protocol] as well as following the [https://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/11_August_2009&action=edit&section=12 changes] which the Metal Sensor Team implemented. Metal sensor team kindly inoculated ''B. subtilis'' cells into flask tubes and placed them into the shaking incubator. The overnight cultures were labelled 1, 2 and 3, while the control was labelled as 4. Our team used overnight culture tube 3 and control tube 4.
Today we are trying to transform ''Bacillus subtilis'' with gfp-rrnb integration vector using the [https://2009.igem.org/Team:Newcastle/Project/Labwork/OurProtocols/TransformBac Transformation Protocol] as well as following the [https://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/11_August_2009&action=edit&section=12 changes] which the Metal Sensor Team implemented. Metal sensor team kindly inoculated ''B. subtilis'' cells into flask tubes and placed them into the shaking incubator. The overnight cultures were labelled 1, 2 and 3, while the control was labelled as 4. Our team used overnight culture tube 3 and control tube 4.
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===Results===
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Following an [https://2009.igem.org/Image:Team_Newcastle_2009_Bacillus_Transformation_Protocol_Steps7-9.PNG illustration] which clearly explains what to plate out from the [https://2009.igem.org/Team:Newcastle/Project/Labwork/OurProtocols/TransformBac Bacillus Transformation Protocol], we plated 4 plates, 3 of which contained the antibiotic chloramphenicol + LB, and 1 containing just LB.
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The plate which contained just LB was the control plate.
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The following pictures show the results of our team's transformations, which were a success.
===Results===
===Results===

Revision as of 02:09, 16 August 2009

Contents

Lab Session 13/08/09

Sporulation Tuning/Chassis Team

Summary

Yesterday's germination attempt seems to have worked, but results are not yet clear. This may have been due to a calculation error for the lysozyme, where instead of adding 40ul of stock lysozyme per ml of buffer solution, 4ul of stock lysozyme was added instead.

Therefore, on Monday, we intend to redo the experiment. See Monday, 17th August for more details.

Today we are trying to transform Bacillus subtilis with gfp-rrnb integration vector using the Transformation Protocol as well as following the changes which the Metal Sensor Team implemented. Metal sensor team kindly inoculated B. subtilis cells into flask tubes and placed them into the shaking incubator. The overnight cultures were labelled 1, 2 and 3, while the control was labelled as 4. Our team used overnight culture tube 3 and control tube 4.

Results

Following an illustration which clearly explains what to plate out from the Bacillus Transformation Protocol, we plated 4 plates, 3 of which contained the antibiotic chloramphenicol + LB, and 1 containing just LB. The plate which contained just LB was the control plate.

The following pictures show the results of our team's transformations, which were a success.

Results



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