Team:Groningen/Notebook/16 July 2009

From 2009.igem.org

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===Vectors===
===Vectors===
 +
 +
ligation of constitutive promotors BBa_J23100, BBa_J23106, BBa_J23109 in vectors pSB1AC3 and pSB3K3.
 +
 +
{|
 +
!
 +
!width="80" align="left"| pSB1AC3 
 +
!width="80" align="left"| pSB3K3
 +
!width="130" align="left"| BBa_J123100
 +
!width="130" align="left"| BBa_J23106
 +
!width="130" align="left"| BBa_J23109
 +
|-
 +
!align="left"|DNA
 +
| 3ul 
 +
| 25ul
 +
| 8,4ul
 +
| 6ul
 +
| 7,4ul
 +
|-
 +
!align="left"| Fast Digest Buffer
 +
| 3ul
 +
| 3ul
 +
| 2ul
 +
| 2ul
 +
| 2ul
 +
|-
 +
!align="left"| EcoRI
 +
| 2ul
 +
| 2uL
 +
| 1ul
 +
| 1ul
 +
| 1ul
 +
|-
 +
!align="left"| SpeI
 +
| 2ul
 +
| 2uL
 +
| 1ul
 +
| 1ul
 +
| 1ul
 +
|-
 +
!align="left"| MQ
 +
| 20ul
 +
|
 +
| 7,6ul
 +
| 10ul
 +
| 8,6ul
 +
|}
 +
 +
* cut for 15 min at 37° , 10 min at 80° heat inactivation
 +
 +
ligation of pSB1AC3 and pSB3K3 with promotors
 +
 +
* 30ul reactions
 +
:: 10ul cut vector
 +
:: 10ul cut promotors
 +
:: 2 ul T4 ligase
 +
:: 3 ul 10x T4 ligation buffer
 +
:: 5 ul MQ
 +
 +
* incubated at 4° overnight
==Dry==
==Dry==

Latest revision as of 15:14, 17 August 2009

[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]

Wet

GVP Cluster

Colony PCR on cells containing the GVP and J23100 ligation product

  • Colonies are picked from the plate with a sterile toothpick and resuspended in 20 μL MQ
  • Mix (1):
  - 2.5 μL Taq buffer (NH4)
  - 0.2 μL dNTP 
  - 2 μL MgCl
  - 16.3 μL MQ
  - 1 μL VF2 primer
  - 1 μL VR primer
  - 1 μL colony solution
  - 1 μL Taq
  • Mix (2):
  - 2.5 μL Taq buffer (KCl)
  - 0.2 μL dNTP 
  - 1.5 μL MgCl
  - 16.8 μL MQ
  - 1 μL VF2 primer
  - 1 μL VR primer
  - 1 μL colony solution
  - 1 μL Taq
PCR program Temperature Time
Denaturing 95° 5.00 min
Start Cycles 20X
Denaturing 95° 30 sec
Annealing 55° 30 sec
Elongation 72° 6.10 min
End cycles
Hold Forever
  • PCR products were analysed on gel (1% agarose, TBE, EtBr, 25 min, 100V)

Results: No bands

Inoculation of Colonies for restriction analysis

  • 3 ml Ty (100 μg/ml Ampicillin) inoculated with colonies from plate (with sterile toothpick)
  • Incubation o/n 37°C in shaker


Transporters

We decided to make MasterMixes of our own. All but 1uL template, 2uL primers and 1uL taq for 25uL reactions.

1X MasterMix Buffer NH SO
Component concentrations volumes
dNTP 0.2 mM 8 uL
MgCl 2.0 mM 80 uL
taq buffer KCl 1X 100 uL
MQ 652 uL
1X MasterMix Buffer KCl
Component concentrations volumes
dNTP 0.2 mM 8 uL
MgCl 1.5 mM 60 uL
taq buffer (NH)SO 1X 100 uL
MQ 672 uL

PCR

We need to have a product with the forward primer. All the other primes give a product. We wil make different PCR product which we can use as mega primers. Second, we will try different PCR programs for example reducing the annealing temperature.

ComponentB1B2C1C2A1A2E1E2F1F2
MM 21222122212221222122
F 1uL FwFwFwFwmut1mut1RevRevFwFw
R 1uL RevRevmut2mut2PCR2PCR2mut1mut1mut1RCmut1RC
DNA 1uL 1010101010
taq 1uL 1111111111
PCR programs B1 B2 C1 C2 A1 A2 E1 E2 F1 F2
ProgramTempTimeTempTimeTempTimeTempTimeTempTime
hotstart955 min955 min955 min955 min955 min
Touchdown50 to 4510X 50 to 4510X 50 to 4510X
Denaturing9530 sec9530 sec9530 sec
annealing5520 sec5520 sec5520 sec
elongations7260 sec7260 sec7260 sec
start cycles ##30X30X25X25X25X
Denaturing9530 sec9530 sec9530 sec9530 sec9530 sec
annealing5520 sec5520 sec5520 sec5520 sec5520 sec
elongations72150 sec72150 sec7260 sec7260 sec7260 sec
end cycles
Final elongation7210 min7210 min7210 min7210 min7210 min
Hold4forever4forever4forever4forever4forever

Metal Accumulation

Vectors

ligation of constitutive promotors BBa_J23100, BBa_J23106, BBa_J23109 in vectors pSB1AC3 and pSB3K3.

pSB1AC3 pSB3K3 BBa_J123100 BBa_J23106 BBa_J23109
DNA 3ul 25ul 8,4ul 6ul 7,4ul
Fast Digest Buffer 3ul 3ul 2ul 2ul 2ul
EcoRI 2ul 2uL 1ul 1ul 1ul
SpeI 2ul 2uL 1ul 1ul 1ul
MQ 20ul 7,6ul 10ul 8,6ul
  • cut for 15 min at 37° , 10 min at 80° heat inactivation

ligation of pSB1AC3 and pSB3K3 with promotors

  • 30ul reactions
10ul cut vector
10ul cut promotors
2 ul T4 ligase
3 ul 10x T4 ligation buffer
5 ul MQ
  • incubated at 4° overnight

Dry

KB extended the Simbiology model to support OpG and OpH in addition to OpN. Jasper in the mean time added some more explanation to the modelling section on the metal accumulation page.

In the end of the afternoon we tried to find more modelling software. Likely candidates are SEMPPR (Japer) and cytoscape (KB) Furthermore KB is trying to get up to date with the paper knowledge.


April
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_April_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
May
MTWTFSS
        [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 6] [http://2009.igem.org/Team:Groningen/Notebook/7_May_2009 7] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 17]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 24]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 27] [http://2009.igem.org/Team:Groningen/Notebook/28_May_2009 28] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/31_May_2009&preload=Team:Groningen/Notebook/Day&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 4] [http://2009.igem.org/Team:Groningen/Notebook/5_June_2009 5] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 7]
[http://2009.igem.org/Team:Groningen/Notebook/8_June_2009 8] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 14]
[http://2009.igem.org/Team:Groningen/Notebook/15_June_2009 15] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 16] [http://2009.igem.org/Team:Groningen/Notebook/17_June_2009 17] [http://2009.igem.org/Team:Groningen/Notebook/18_June_2009 18] [http://2009.igem.org/Team:Groningen/Notebook/19_June_2009 19] [http://2009.igem.org/Team:Groningen/Notebook/20_June_2009 20] [http://2009.igem.org/Team:Groningen/Notebook/21_June_2009 21]
[http://2009.igem.org/Team:Groningen/Notebook/22_June_2009 22] [http://2009.igem.org/Team:Groningen/Notebook/23_June_2009 23] [http://2009.igem.org/Team:Groningen/Notebook/24_June_2009 24] [http://2009.igem.org/Team:Groningen/Notebook/25_June_2009 25] [http://2009.igem.org/Team:Groningen/Notebook/26_June_2009 26] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_June_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]
July
MTWTFSS
    [http://2009.igem.org/Team:Groningen/Notebook/1_July_2009 1] [http://2009.igem.org/Team:Groningen/Notebook/2_July_2009 2] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 5]
[http://2009.igem.org/Team:Groningen/Notebook/6_July_2009 6] [http://2009.igem.org/Team:Groningen/Notebook/7_July_2009 7] [http://2009.igem.org/Team:Groningen/Notebook/8_July_2009 8] [http://2009.igem.org/Team:Groningen/Notebook/9_July_2009 9] [http://2009.igem.org/Team:Groningen/Notebook/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 12]
[http://2009.igem.org/Team:Groningen/Notebook/13_July_2009 13] [http://2009.igem.org/Team:Groningen/Notebook/14_July_2009 14] [http://2009.igem.org/Team:Groningen/Notebook/15_July_2009 15] [http://2009.igem.org/Team:Groningen/Notebook/16_July_2009 16] [http://2009.igem.org/Team:Groningen/Notebook/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 19]
[http://2009.igem.org/Team:Groningen/Notebook/20_July_2009 20] [http://2009.igem.org/Team:Groningen/Notebook/21_July_2009 21] [http://2009.igem.org/Team:Groningen/Notebook/22_July_2009 22] [http://2009.igem.org/Team:Groningen/Notebook/23_July_2009 23] [http://2009.igem.org/Team:Groningen/Notebook/24_July_2009 24] [http://2009.igem.org/Team:Groningen/Notebook/25_July_2009 25] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_July_2009&preload=Team:Groningen/Notebook/Day&action=edit 26]
[http://2009.igem.org/Team:Groningen/Notebook/27_July_2009 27] [http://2009.igem.org/Team:Groningen/Notebook/28_July_2009 28] [http://2009.igem.org/Team:Groningen/Notebook/29_July_2009 29] [http://2009.igem.org/Team:Groningen/Notebook/30_July_2009 30] [http://2009.igem.org/Team:Groningen/Notebook/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/Team:Groningen/Notebook/1_August_2009 1] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_August_2009&preload=Team:Groningen/Notebook/Day&action=edit 2]
[http://2009.igem.org/Team:Groningen/Notebook/3_August_2009 3] [http://2009.igem.org/Team:Groningen/Notebook/4_August_2009 4] [http://2009.igem.org/Team:Groningen/Notebook/5_August_2009 5] [http://2009.igem.org/Team:Groningen/Notebook/6_August_2009 6] [http://2009.igem.org/Team:Groningen/Notebook/7_August_2009 7] [http://2009.igem.org/Team:Groningen/Notebook/8_August_2009 8] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_August_2009&preload=Team:Groningen/Notebook/Day&action=edit 9]
[http://2009.igem.org/Team:Groningen/Notebook/10_August_2009 10] [http://2009.igem.org/Team:Groningen/Notebook/11_August_2009 11] [http://2009.igem.org/Team:Groningen/Notebook/12_August_2009 12] [http://2009.igem.org/Team:Groningen/Notebook/13_August_2009 13] [http://2009.igem.org/Team:Groningen/Notebook/14_August_2009 14] [http://2009.igem.org/Team:Groningen/Notebook/15_August_2009 15] [http://2009.igem.org/Team:Groningen/Notebook/16_August_2009 16]
[http://2009.igem.org/Team:Groningen/Notebook/17_August_2009 17] [http://2009.igem.org/Team:Groningen/Notebook/18_August_2009 18] [http://2009.igem.org/Team:Groningen/Notebook/19_August_2009 19] [http://2009.igem.org/Team:Groningen/Notebook/20_August_2009 20] [http://2009.igem.org/Team:Groningen/Notebook/21_August_2009 21] [http://2009.igem.org/Team:Groningen/Notebook/22_August_2009 22] [http://2009.igem.org/Team:Groningen/Notebook/23_August_2009 23]
[http://2009.igem.org/Team:Groningen/Notebook/24_August_2009 24] [http://2009.igem.org/Team:Groningen/Notebook/25_August_2009 25] [http://2009.igem.org/Team:Groningen/Notebook/26_August_2009 26] [http://2009.igem.org/Team:Groningen/Notebook/27_August_2009 27] [http://2009.igem.org/Team:Groningen/Notebook/28_August_2009 28] [http://2009.igem.org/Team:Groningen/Notebook/29_August_2009 29] [http://2009.igem.org/Team:Groningen/Notebook/30_August_2009 30]
[http://2009.igem.org/Team:Groningen/Notebook/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Team:Groningen/Notebook/1_September_2009 1] [http://2009.igem.org/Team:Groningen/Notebook/2_September_2009 2] [http://2009.igem.org/Team:Groningen/Notebook/3_September_2009 3] [http://2009.igem.org/Team:Groningen/Notebook/4_September_2009 4] [http://2009.igem.org/Team:Groningen/Notebook/5_September_2009 5] [http://2009.igem.org/Team:Groningen/Notebook/6_September_2009 6]
[http://2009.igem.org/Team:Groningen/Notebook/7_September_2009 7] [http://2009.igem.org/Team:Groningen/Notebook/8_September_2009 8] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 9] [http://2009.igem.org/Team:Groningen/Notebook/10_September_2009 10] [http://2009.igem.org/Team:Groningen/Notebook/11_September_2009 11] [http://2009.igem.org/Team:Groningen/Notebook/12_September_2009 12] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 13]
[http://2009.igem.org/Team:Groningen/Notebook/14_September_2009 14] [http://2009.igem.org/Team:Groningen/Notebook/15_September_2009 15] [http://2009.igem.org/Team:Groningen/Notebook/16_September_2009 16] [http://2009.igem.org/Team:Groningen/Notebook/17_September_2009 17] [http://2009.igem.org/Team:Groningen/Notebook/18_September_2009 18] [http://2009.igem.org/Team:Groningen/Notebook/19_September_2009 19] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 20]
[http://2009.igem.org/Team:Groningen/Notebook/21_September_2009 21] [http://2009.igem.org/Team:Groningen/Notebook/22_September_2009 22] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 23] [http://2009.igem.org/Team:Groningen/Notebook/24_September_2009 24] [http://2009.igem.org/Team:Groningen/Notebook/25_September_2009 25] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_September_2009&preload=Team:Groningen/Notebook/Day&action=edit 27]
[http://2009.igem.org/Team:Groningen/Notebook/28_September_2009 28] [http://2009.igem.org/Team:Groningen/Notebook/29_September_2009 29] [http://2009.igem.org/Team:Groningen/Notebook/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 1] [http://2009.igem.org/Team:Groningen/Notebook/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 4]
[http://2009.igem.org/Team:Groningen/Notebook/5_October_2009 5] [http://2009.igem.org/Team:Groningen/Notebook/6_October_2009 6] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 11]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/14_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/17_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 18]
[http://2009.igem.org/Team:Groningen/Notebook/19_October_2009 19] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/31_October_2009&preload=Team:Groningen/Notebook/Day&action=edit 31]
November
MTWTFSS
            [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/1_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 1]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/2_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/3_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/4_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/5_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/6_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/7_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/8_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 8]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/9_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/10_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/11_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/12_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/13_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 13] [http://2009.igem.org/Team:Groningen/Notebook/14_November_2009 14] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/15_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 15]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/16_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 16] [http://2009.igem.org/Team:Groningen/Notebook/17_November_2009 17] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/18_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/19_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/20_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/21_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/22_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 22]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/23_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/24_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/25_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/26_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/27_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/28_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/29_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 29]
[http://2009.igem.org/wiki/index.php?title=Team:Groningen/Notebook/30_November_2009&preload=Team:Groningen/Notebook/Day&action=edit 30]