Team:Newcastle/Labwork/23 July 2009

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# [http://partsregistry.org/Part:BBa_C0076 BBa_C0076] – Autoinducer synthetase
# [http://partsregistry.org/Part:BBa_C0076 BBa_C0076] – Autoinducer synthetase
# [http://partsregistry.org/Part:BBa_R0077 BBa_R0077] – Promoter (CinR and HSL regulated, RBS+)
# [http://partsregistry.org/Part:BBa_R0077 BBa_R0077] – Promoter (CinR and HSL regulated, RBS+)
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===Practical Outline===
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# <font color="orange">Precipitate the ethanol from our DNA plasmids (containing the RFP and GFP genes) and analyse them through DNA gel electrophoresis - this is Introductory Lab Session work</font>
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# Grow up some ''DH5alpha E.coli'' cells containing the ''pMUTIN4'' plasmid vector
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# Hydrate and prepare BioBricks present in Spring Distributions.
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Revision as of 17:08, 18 August 2009

Lab Work - 23/07/09

Introduction

Today we will carry out one of the last tasks remaining from our practice lab sessions - precipitating the ethanol from our DNA samples so that gel electrophoresis can be carried out. This can be seen at this link - Introductory Lab Session (23/07/09).

Today also marks the beginning of the real lab work sessions in which the work will be directly involved in implementing our system. The first real task involves growing up E.coli cultures containing the plasmid backbone pMUTIN4, which will allow us in the near future to transfer our chosen BioBricks from the E.coli cells we are working with into Bacillus subtilis.

Another task which we have to complete is to prepare the BioBricks which are needed for our system and are found in the Spring Distributions. These include:

  1. [http://partsregistry.org/Part:BBa_C0056 BBa_C0056] – cI repressor (lambda phage)
  2. [http://partsregistry.org/Part:BBa_B1002 BBa_B1002] – Terminator sequence
  3. [http://partsregistry.org/Part:BBa_C0077 BBa_C0077] – CinR activator
  4. [http://partsregistry.org/Part:BBa_C0076 BBa_C0076] – Autoinducer synthetase
  5. [http://partsregistry.org/Part:BBa_R0077 BBa_R0077] – Promoter (CinR and HSL regulated, RBS+)

Practical Outline

  1. Precipitate the ethanol from our DNA plasmids (containing the RFP and GFP genes) and analyse them through DNA gel electrophoresis - this is Introductory Lab Session work
  2. Grow up some DH5alpha E.coli cells containing the pMUTIN4 plasmid vector
  3. Hydrate and prepare BioBricks present in Spring Distributions.



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