Team:Newcastle/Labwork/20 August 2009

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In yesterday's lab session, the metal sensor team inoculated 3 tubes of 3ml LB with the three transformant ''E.coli'' cultures grown on the LB + ampicillin plates (i.e. the ''E.coli'' bacteria which had taken up the ''BBa_J33206'' DNA). In addition, 0.5ml of each of these three solutions was used to innoculate 3 flasks containing 50ml LB each. The initial inoculations of 3ml LB solutions is for today's mini prep; the subsequent inoculations of 50ml LB solutions is for today's midi prep.
In yesterday's lab session, the metal sensor team inoculated 3 tubes of 3ml LB with the three transformant ''E.coli'' cultures grown on the LB + ampicillin plates (i.e. the ''E.coli'' bacteria which had taken up the ''BBa_J33206'' DNA). In addition, 0.5ml of each of these three solutions was used to innoculate 3 flasks containing 50ml LB each. The initial inoculations of 3ml LB solutions is for today's mini prep; the subsequent inoculations of 50ml LB solutions is for today's midi prep.
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Today, the team hope to '''firstly carry out a mini prep''' for the three transformant cultures. '''Secondly, it is intended that restriction enzyme digests are carried out on a sample of all three culture plasmids along with DNA gel electrophoresis''' to determine whether the transformed DNA is in fact the ''BBa_J33206'' BioBrick. Thirdly, if time permits, ''the team hope to carry out a midi prep of the plasmids''' for later cloning. If all of these steps do not get completed today, they can resumed tomorrow.
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Today, the team hope to '''firstly carry out a mini prep''' for the three transformant cultures. '''Secondly, it is intended that restriction enzyme digests are carried out on a sample of all three culture plasmids along with DNA gel electrophoresis''' to determine whether the transformed DNA is in fact the ''BBa_J33206'' BioBrick. Thirdly, if time permits, '''the team hope to carry out a midi prep of the plasmids''' for later cloning. If all of these steps do not get completed today, they can resumed tomorrow.
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Revision as of 20:51, 23 August 2009

Lab 20/08/09

Metal Sensing Team

Introduction

In yesterday's lab session, the metal sensor team inoculated 3 tubes of 3ml LB with the three transformant E.coli cultures grown on the LB + ampicillin plates (i.e. the E.coli bacteria which had taken up the BBa_J33206 DNA). In addition, 0.5ml of each of these three solutions was used to innoculate 3 flasks containing 50ml LB each. The initial inoculations of 3ml LB solutions is for today's mini prep; the subsequent inoculations of 50ml LB solutions is for today's midi prep.

Today, the team hope to firstly carry out a mini prep for the three transformant cultures. Secondly, it is intended that restriction enzyme digests are carried out on a sample of all three culture plasmids along with DNA gel electrophoresis to determine whether the transformed DNA is in fact the BBa_J33206 BioBrick. Thirdly, if time permits, the team hope to carry out a midi prep of the plasmids for later cloning. If all of these steps do not get completed today, they can resumed tomorrow.



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