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Team:Groningen/Notebook/27 August 2009
From 2009.igem.org
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===GVP Cluster=== | ===GVP Cluster=== | ||
| - | :→ {{ | + | :→ {{done}} Check o.n. plates with transformed E.coli TOP10 cells |
:→ {{todo}} Use colonies to grow o.n. precultures for plasmid isolation | :→ {{todo}} Use colonies to grow o.n. precultures for plasmid isolation | ||
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|27/8 | |27/8 | ||
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| + | :→ The plates where old DNA fragments were used showed a lower amount of colonies, probably due to degradation of the DNA ends. In the case of the constitutive promoter BBa_J23109 no colonies at all were visible, and can be explained by both fragments being cut earlier. | ||
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| + | :→ From the "new" plates four colonies of each construct will be used for preculture growth, and from the "old" fragments only two will be used. (12 in total) | ||
===Transporters=== | ===Transporters=== | ||
Revision as of 07:53, 27 August 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
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Wet
GVP Cluster
- → DONE Check o.n. plates with transformed E.coli TOP10 cells
- → TODO Use colonies to grow o.n. precultures for plasmid isolation
- → TODO Test promoter strenght compared to BBa_J23101 promoter (Sven)
- → TODO Enter sequences of constructs to Sandbox
Colonies on Plates
| Name | Plasmid Used | Antibiotics on Plasmid | No. of Colonies | Date |
| LAC-GVP (new) | pSB1AC3 | Ampicillin | ~80 | 27/8 |
| LAC-GVP (new, concentrated) | pSB1AC3 | Ampicillin | ~200 | 27/8 |
| LAC-GVP (old) | pSB1AC3 | Ampicillin | 3 | 27/8 |
| LAC-GVP (old, concentrated) | pSB1AC3 | Ampicillin | 11 | 27/8 |
| pBAD-GVP (new) | pSB1AC3 | Ampicillin | 18 | 27/8 |
| pBAD-GVP (new, concentrated) | pSB1AC3 | Ampicillin | ~80 | 27/8 |
| pBAD-GVP (old) | pSB1AC3 | Ampicillin | 4 | 27/8 |
| pBAD-GVP (old, concentrated) | pSB1AC3 | Ampicillin | 28 | 27/8 |
| pLow (constitutive)-GVP (old) | pSB3K3 | Kanamycin | 0 | 27/8 |
| pLow (constitutive)-GVP (old, concentrated) | pSB3K3 | Kanamycin | 0 | 27/8 |
| MQ (neg. control) | X | X | 0 | 27/8 |
| pHigh (constitutive)-RFP (pos. control) | J61002 | Ampicillin | ~2000 | 27/8 |
- → The plates where old DNA fragments were used showed a lower amount of colonies, probably due to degradation of the DNA ends. In the case of the constitutive promoter BBa_J23109 no colonies at all were visible, and can be explained by both fragments being cut earlier.
- → From the "new" plates four colonies of each construct will be used for preculture growth, and from the "old" fragments only two will be used. (12 in total)
Transporters
Metal Accumulation
Vectors
Dry
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