Virginia Commonwealth/4 June 2009
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==Thursday 04 June 2009== | ==Thursday 04 June 2009== | ||
===Results=== | ===Results=== | ||
+ | *Overnight 5mL culture of I13521 looks great (i.e., it's pinkish red) - [[User:GMcArthurIV|GMcArthurIV]] 15:03, 4 June 2009 (UTC) | ||
===Tasks=== | ===Tasks=== | ||
*Lab orientation and safety overview | *Lab orientation and safety overview |
Revision as of 15:03, 4 June 2009
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Contents |
Thursday 04 June 2009
Results
- Overnight 5mL culture of I13521 looks great (i.e., it's pinkish red) - GMcArthurIV 15:03, 4 June 2009 (UTC)
Tasks
- Lab orientation and safety overview
- Synthetic biology overview discussion
- Literature discussion
Wetlab
- Introduction to media making
- Transform NEB10β or DB3.1 with the following parts:
Part No. | Type | ccdB gene | Resistance | Plate | Well | |
---|---|---|---|---|---|---|
[http://partsregistry.org/Part:BBa_R0040 R0040] | Promoter | No | AmpR | N/A | N/A | |
[http://partsregistry.org/Part:BBa_E0840 E0840] | GFP RET | No | AmpR | N/A | N/A | |
[http://partsregistry.org/Part:BBa_J13002 J13002] | Expression plasmid | No | AmpR | 1 | 13B | |
[http://partsregistry.org/Part:BBa_E1010 E1010] | RFP CDS | No | KanR | 1 | 18F | |
[http://partsregistry.org/wiki/index.php?title=Part:pSB1C3 pSB1C3] | High-copy assembly plasmid | No | CmR | 1 | 5A | |
[http://partsregistry.org/wiki/index.php?title=Part:pSB4C5 pSB4C5] | Low-copy assembly plasmid | Yes | CmR | 1 | 5I |
- Run gel of single and double digests on I13521 (George and Chris)