Template:Team:KULeuven/24 August 2009/BlueLightReceptor

From 2009.igem.org

(Difference between revisions)
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# plates with ligA were put under blue light. the LEDs were put on their max capacity.  
# plates with ligA were put under blue light. the LEDs were put on their max capacity.  
# restriction digest with  
# restriction digest with  
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#*tubes (1,3,5,7,9) of ligA (BLP + E0240) cut with EcoRI en PstI
+
#*tubes (1,3,5,7,9) of ligA (BLP + {{kulpart|BBa_E0240}}) cut with EcoRI en PstI
-
#*promotor J23101 cut with EcoRI en SpeI (4x)
+
#*promotor {{kulpart|BBa_J23101}} cut with EcoRI en SpeI (4x)
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#gel electroforese with the RD of ligA and J23101 followed by a gel extraction:
+
#gel electroforese with the RD of ligA and {{kulpart|BBa_J23101}} followed by a gel extraction:
#* note: tube 5 of ligA was loaded poorly on the gel and could not be used.
#* note: tube 5 of ligA was loaded poorly on the gel and could not be used.
-
#* note: the samples of promotor J23101 were barly visible. only 2 of the 4 samples were recoverd by extraction.  
+
#* note: the samples of promotor {{kulpart|BBa_J23101}} were barly visible. only 2 of the 4 samples were recoverd by extraction.  
{| border ="1" align="center"
{| border ="1" align="center"
!| Part || concentration (ng/μl) || 260/280 λ ||  
!| Part || concentration (ng/μl) || 260/280 λ ||  
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| {{kulpart|BBa_J23101}} (B) ||12,8|| 2,57 ||
| {{kulpart|BBa_J23101}} (B) ||12,8|| 2,57 ||
|}
|}
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4. enting of liquid cultures with kanamycin and pSB3K3
+
4. enting of liquid cultures with kanamycin and {{kulpart|pSB3K3}}

Revision as of 11:53, 1 September 2009

  1. plates with ligA were put under blue light. the LEDs were put on their max capacity.
  2. restriction digest with
    • tubes (1,3,5,7,9) of ligA (BLP + ) cut with EcoRI en PstI
    • promotor cut with EcoRI en SpeI (4x)
  3. gel electroforese with the RD of ligA and followed by a gel extraction:
    • note: tube 5 of ligA was loaded poorly on the gel and could not be used.
    • note: the samples of promotor were barly visible. only 2 of the 4 samples were recoverd by extraction.
Part concentration (ng/μl) 260/280 λ
ligA 1 7,9 2,16
ligA 3 10,8 1,88
ligA 7 4,8 2,49
ligA 9 8,0 3,02
(A) 20,8 1,70
(B) 12,8 2,57

4. enting of liquid cultures with kanamycin and

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