Team:Newcastle/Project/Labwork/OurProtocols/PCR Products

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(New page: *Use PCR Clean-up kit to clear the PCR product This should result in 50ul of final product *Measure the concentration of the product This step will will be useful to determine how much of ...)
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*Use PCR Clean-up kit to clear the PCR product
*Use PCR Clean-up kit to clear the PCR product
This should result in 50ul of final product
This should result in 50ul of final product
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*Digest the PCR product
*Digest the PCR product
Although the total will be 50ul, some of it will be consumed for the gel and to measure the concentration
Although the total will be 50ul, some of it will be consumed for the gel and to measure the concentration
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Revision as of 14:24, 1 September 2009

  • Use PCR Clean-up kit to clear the PCR product

This should result in 50ul of final product

  • Measure the concentration of the product

This step will will be useful to determine how much of the product to use for the ligation

  • Digest the PCR product

Although the total will be 50ul, some of it will be consumed for the gel and to measure the concentration



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