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EPF-Lausanne/28 August 2009
From 2009.igem.org
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==Wet Lab== | ==Wet Lab== | ||
We did a '''glycerol stock''' out of the 3 LR2+GFP clones : LacI-RBS #1, #2 #4 + GFP (E02110). | We did a '''glycerol stock''' out of the 3 LR2+GFP clones : LacI-RBS #1, #2 #4 + GFP (E02110). | ||
| + | |||
| + | ===Characterisation of RO2 + LacI-RBS=== | ||
| + | We used the reader in Sebastian's lab (qPCR machine). | ||
| + | |||
| + | We took 3 clones of RO2 (3 in M9, 3 in LB), and put 50 ul in each well. In 3 wells we add ATC (5ul), in 3 other nothing, in the next 3 TRP and finally in the last 3 IPTG (to induce LacI). | ||
==People in the lab== | ==People in the lab== | ||
Revision as of 10:04, 4 September 2009
Wet Lab
We did a glycerol stock out of the 3 LR2+GFP clones : LacI-RBS #1, #2 #4 + GFP (E02110).
Characterisation of RO2 + LacI-RBS
We used the reader in Sebastian's lab (qPCR machine).
We took 3 clones of RO2 (3 in M9, 3 in LB), and put 50 ul in each well. In 3 wells we add ATC (5ul), in 3 other nothing, in the next 3 TRP and finally in the last 3 IPTG (to induce LacI).
People in the lab
Basile, Rafael, Nicolas
