EPF-Lausanne/28 August 2009

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Revision as of 10:08, 4 September 2009

Wet Lab

We did a glycerol stock out of the 3 LR2+GFP clones : LacI-RBS #1, #2 #4 + GFP (E02110).

Characterisation of RO2 + LacI-RBS

We used the reader in Sebastian's lab (qPCR machine).

We took 3 clones of RO2 (3 in M9, 3 in LB), and put 50 ul in each well. In 3 wells we add ATC (5 ul), in 3 other nothing, in the next 3 TRP (5 ul) and finally in the last 3 IPTG (5 ul, to induce LacI).

It was put for 2 hours at 37°C and 1 picture was taken each 3 minutes.

The results weren't concluding :

- All clones with ATC (no matter M9 or LB) had a "linear" progression.

- The clones (M9 and LB) with nothing and TRP didn't make any difference (linear half way then growing a little bit exponentially).

- LacI-RBS-GFP clones didn't seem to react to the IPTG induction (same pattern as those which hadn't been induced).

Analysis of the results

People in the lab

Basile, Rafael, Nicolas

@@ Line 31: / Line 31: @@
 We used the reader in Sebastian's lab (qPCR machine).
-We took 3 clones of RO2 (3 in M9, 3 in LB), and put 50 ul in each well. In 3 wells we add ATC (5ul), in 3 other nothing, in the next 3 TRP and finally in the last 3 IPTG (to induce LacI).
+We took 3 clones of RO2 (3 in M9, 3 in LB), and put 50 ul in each well. In 3 wells we add ATC (5 ul), in 3 other nothing, in the next 3 TRP (5 ul) and finally in the last 3 IPTG (5 ul, to induce LacI).
+It was put for 2 hours at 37°C and 1 picture was taken each 3 minutes.
+The '''results''' weren't concluding :
+- All clones with ATC (no matter M9 or LB) had a "linear" progression.
+- The clones (M9 and LB) with nothing and TRP didn't make any difference (linear half way then growing a little bit exponentially).
+- LacI-RBS-GFP clones didn't seem to react to the IPTG induction (same pattern as those which hadn't been induced).
+'''Analysis of the results'''
 ==People in the lab==