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EPF-Lausanne/29 August 2009
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==Wet Lab== | ==Wet Lab== | ||
| + | '''Glycerol stock''' of the following clones : | ||
| + | -BB5-RO2#5, clone #1 ("double" construction) | ||
| + | |||
| + | -BB5-RO2#5, clone #4 ("double" construction) | ||
| + | |||
| + | -RO2#4 + BB1, clone #3 (double transformation) | ||
| + | |||
| + | -RO2#10 + BB6, clone #8 (double transformation) | ||
| + | |||
| + | They were put in the -80°C freezer (2nd box). | ||
| + | |||
| + | Although so far the colony PCR on these RO + BB didn't show anything concluding, the fact that they grew in antibiotic selection seems to show that it actually worked. | ||
| + | |||
| + | We also re-plated the double transformation clones on newly made plates containing both AMP and KANA. It grew, so it seems to indicate that what we have is right. | ||
==People in the lab== | ==People in the lab== | ||
Revision as of 10:29, 4 September 2009
Contents |
Wet Lab
Glycerol stock of the following clones :
-BB5-RO2#5, clone #1 ("double" construction)
-BB5-RO2#5, clone #4 ("double" construction)
-RO2#4 + BB1, clone #3 (double transformation)
-RO2#10 + BB6, clone #8 (double transformation)
They were put in the -80°C freezer (2nd box).
Although so far the colony PCR on these RO + BB didn't show anything concluding, the fact that they grew in antibiotic selection seems to show that it actually worked.
We also re-plated the double transformation clones on newly made plates containing both AMP and KANA. It grew, so it seems to indicate that what we have is right.
People in the lab
Basile (Saturday !)
