Team:Newcastle/Labwork/24 August 2009

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=Lab Work - 24/08/09=
==<u>Stochastic Switch team</u>==
==<u>Stochastic Switch team</u>==
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[[Image:Team Newcastle 2009 iGEM 24-08-09 IMG 0519.JPG|200px|thumb|Jess preparing to innoculate 3ml of LB with some transformant cultures]]
===Summary===
===Summary===
Today was dedicated to finishing our cloning strategies and primer designs, and so lab was called off. However the stochastic switch team had time to set up some overnight cultures to redo the minipreps and restriction digests for the biobricks from last week. These will be carried out tomorrow. We mixed the colonies in 3 ml of LB+Amp and left them overnight in shaking incubator. To prepare LB + Amp solution we used 5ml of LB + 250ul of Amp. 12 cultures were left overnight.
Today was dedicated to finishing our cloning strategies and primer designs, and so lab was called off. However the stochastic switch team had time to set up some overnight cultures to redo the minipreps and restriction digests for the biobricks from last week. These will be carried out tomorrow. We mixed the colonies in 3 ml of LB+Amp and left them overnight in shaking incubator. To prepare LB + Amp solution we used 5ml of LB + 250ul of Amp. 12 cultures were left overnight.

Revision as of 00:37, 7 September 2009

Lab Work - 24/08/09

Stochastic Switch team

Jess preparing to innoculate 3ml of LB with some transformant cultures

Summary

Today was dedicated to finishing our cloning strategies and primer designs, and so lab was called off. However the stochastic switch team had time to set up some overnight cultures to redo the minipreps and restriction digests for the biobricks from last week. These will be carried out tomorrow. We mixed the colonies in 3 ml of LB+Amp and left them overnight in shaking incubator. To prepare LB + Amp solution we used 5ml of LB + 250ul of Amp. 12 cultures were left overnight.



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