EPF-Lausanne/3 September 2009
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==Wet Lab== | ==Wet Lab== | ||
+ | * '''Results of the RO1:''' | ||
+ | <br>There is clones on every plate! | ||
+ | : → colony PCR on all clones with th eTaq Platinium protocol | ||
+ | |||
+ | * '''Results from the media try:''' | ||
+ | <br>All media have worked! (except for the SC) They have different colors depending on media and clone after an overnight culture. | ||
+ | |||
+ | {| class="wikitable" style="text-align:center; width:80%;" | ||
+ | |- | ||
+ | ! scope=col | | ||
+ | ! scope=col | RO2 #6 | ||
+ | ! scope=col | RO2 #7 | ||
+ | ! scope=col | RO2 #8 | ||
+ | |- | ||
+ | ! scope=row | M9/min + AA + glycerol | ||
+ | |white | ||
+ | |white | ||
+ | |white | ||
+ | |- | ||
+ | ! scope=row | LB | ||
+ | |LB + slight red | ||
+ | |LB + red | ||
+ | |LB | ||
+ | |- | ||
+ | ! scope=row | 9/min + AA + thiamine | ||
+ | |rose | ||
+ | |white | ||
+ | |white | ||
+ | |- | ||
+ | ! scope=row | 9/min + thiamine | ||
+ | |rose | ||
+ | |rose | ||
+ | |white | ||
+ | |- | ||
+ | ! scope=row | SC | ||
+ | |NA | ||
+ | |NA | ||
+ | |NA | ||
+ | |} | ||
+ | |||
+ | |||
Revision as of 08:13, 7 September 2009
Contents |
Wet Lab
- Results of the RO1:
There is clones on every plate!
- → colony PCR on all clones with th eTaq Platinium protocol
- Results from the media try:
All media have worked! (except for the SC) They have different colors depending on media and clone after an overnight culture.
RO2 #6 | RO2 #7 | RO2 #8 | |
---|---|---|---|
M9/min + AA + glycerol | white | white | white |
LB | LB + slight red | LB + red | LB |
9/min + AA + thiamine | rose | white | white |
9/min + thiamine | rose | rose | white |
SC | NA | NA | NA |
People in the lab
Basile, Mélanie, Caroline