EPF-Lausanne/3 September 2009

From 2009.igem.org

(Difference between revisions)
(People in the lab)
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==Wet Lab==
==Wet Lab==
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* '''Results of the RO1:'''
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<br>There is clones on every plate!
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: → colony PCR on all clones with th eTaq Platinium protocol
 +
 +
* '''Results from the media try:'''
 +
<br>All media have worked! (except for the SC) They have different colors depending on media and clone after an overnight culture.
 +
 +
{| class="wikitable" style="text-align:center; width:80%;"
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|-
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! scope=col |
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! scope=col | RO2 #6
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! scope=col | RO2 #7
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! scope=col | RO2 #8
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|-
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! scope=row | M9/min + AA + glycerol
 +
|white
 +
|white
 +
|white
 +
|-
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! scope=row | LB
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|LB + slight red
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|LB + red
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|LB
 +
|-
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! scope=row | 9/min + AA + thiamine
 +
|rose
 +
|white
 +
|white
 +
|-
 +
! scope=row | 9/min + thiamine
 +
|rose
 +
|rose
 +
|white
 +
|-
 +
! scope=row | SC
 +
|NA
 +
|NA
 +
|NA
 +
|}
 +
 +

Revision as of 08:13, 7 September 2009

Contents

3 September 2009





Wet Lab

  • Results of the RO1:


There is clones on every plate!

→ colony PCR on all clones with th eTaq Platinium protocol
  • Results from the media try:


All media have worked! (except for the SC) They have different colors depending on media and clone after an overnight culture.

RO2 #6 RO2 #7 RO2 #8
M9/min + AA + glycerol white white white
LB LB + slight red LB + red LB
9/min + AA + thiamine rose white white
9/min + thiamine rose rose white
SC NA NA NA



People in the lab

Basile, Mélanie, Caroline