Uppsala-Sweden/7 September 2009

From 2009.igem.org

(Difference between revisions)
Line 48: Line 48:
Report for the above preps: [[Media:Plasmidprep_090907.txt]]
Report for the above preps: [[Media:Plasmidprep_090907.txt]]
--[[User:Anders|Anders]] 17:49, 7 September 2009 (UTC)
--[[User:Anders|Anders]] 17:49, 7 September 2009 (UTC)
 +
 +
==2nd Plasmid Prep==
 +
 +
Prep of the B0015 (AC3) culture from this morning
 +
 +
--[[User:Karl.brune|Karl.brune]] 21:50, 7 September 2009 (UTC)
 +
 +
==Pouring Plates==
 +
 +
I poured plates ( Kanamycin and Chloramphenicol ), stored at iGEM fridge
 +
 +
--[[User:Karl.brune|Karl.brune]] 21:50, 7 September 2009 (UTC)
 +
==Inoculation for PP==
 +
 +
#pSB1A3
 +
#pSB1AC3 (2)
 +
#pSB1AK3 (2)
 +
#B0034 (A) (2)
 +
#I13504 (A) (2)
 +
 +
Overnight at 37C, 250RPM in 6ml LB with specific antitbiotic
 +
 +
--[[User:Karl.brune|Karl.brune]] 21:50, 7 September 2009 (UTC)

Revision as of 21:50, 7 September 2009




Contents

Plasmid Preps

Plasmid Prep with Sigma Gene Elute was performed with the following samples:

Subunit E2

  1. E21-1
  2. E22-3
  3. E23-3
  4. E24-1

Subunit E1 alpha

  1. E1A1-1
  2. E1A1-2
  3. E1A2-2
  4. E1A2-3
  5. E1A3-1
  6. E1A4-2

Subunit E1 beta

  1. E1B1-3
  2. E1B2-3
  3. E1B3-1
  4. E1B4-2

B0015, B0034-pirA, R0011-B0034, B0034-Yadh2-B0015

  1. T-1 - inoculation failed, new inoculation was performed
  2. RP-2
  3. RR-4
  4. RR-5
  5. RYT-1
  6. RYT-2

Furthermore these were prepped (Lux System etc.)

A340620, C0621, J237034

  1. A340620
  2. C0621
  3. J237034

and these here:

  1. R0062 (A)
  2. RARP 4, 5 ,7 (CH) - Erik performed these, please update Notebook

--Karl.brune 14:12, 7 September 2009 (UTC)

Report for the above preps: Media:Plasmidprep_090907.txt --Anders 17:49, 7 September 2009 (UTC)

2nd Plasmid Prep

Prep of the B0015 (AC3) culture from this morning

--Karl.brune 21:50, 7 September 2009 (UTC)

Pouring Plates

I poured plates ( Kanamycin and Chloramphenicol ), stored at iGEM fridge

--Karl.brune 21:50, 7 September 2009 (UTC)

Inoculation for PP

  1. pSB1A3
  2. pSB1AC3 (2)
  3. pSB1AK3 (2)
  4. B0034 (A) (2)
  5. I13504 (A) (2)

Overnight at 37C, 250RPM in 6ml LB with specific antitbiotic

--Karl.brune 21:50, 7 September 2009 (UTC)

Retrieved from "http://2009.igem.org/Uppsala-Sweden/7_September_2009"