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Template:Team:KULeuven/5 August 2009/VanillinProduction
From 2009.igem.org
(Difference between revisions)
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C2: '''Ech''', EcoRI en XbaI<br> | C2: '''Ech''', EcoRI en XbaI<br> | ||
| - | The mixture was incubated in | + | The mixture was incubated in 37°C for 1h30... |
| - | + | ...followed by gelelectrophoresis of the digested parts | |
Revision as of 08:16, 8 September 2009
Miniprep of pairs:
- RBS + Sam5 conc: 101,3 ng/ul
- RBS+Sam8 conc: 95.7 ng/ul
- Sam8 conc: 81,8 ng/ul
- ComT , conc: 122,9 ng/ul
Restriction digestion
A1: Sam8, EcoRI en SpeI
A2: Sam5, EcoRI en XbaI
B1: RBS, EcoRI en SpeI
B2: ComT, EcoRI en XbaI
C1: Fcs, EcoRI en SpeI
C2: Ech, EcoRI en XbaI
The mixture was incubated in 37°C for 1h30...
...followed by gelelectrophoresis of the digested parts
