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Team:UNIPV-Pavia/Notebook/Week1Sep
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== <html><font class="dayw_style">September, 1st</font></html> == | == <html><font class="dayw_style">September, 1st</font></html> == | ||
| + | *We received sequencing results for: | ||
| + | ** | ||
| + | ** | ||
| + | ** | ||
| + | ** | ||
| + | *Glycerol stocks for the 10 B5new2 grown cultures. | ||
| + | |||
| + | *Miniprep for B5new2 (10 samples) and for B8-5. | ||
| + | |||
| + | *Digestion for: | ||
| + | **B5new2 (10 samples) for screening (E-P cut); | ||
| + | **B8-5 for purification from gel (E-P cut); | ||
| + | **R0011 (stored at -20°C) for A17new ligation (S-P cut) | ||
| + | **E0240 (stored at -20°C) for A17new ligation (X-P cut) | ||
| + | |||
| + | *Medium-size gel for B5new2(E-P); small-size gel for B8-5(E-P), R0011(S-P) and E0240(X-P). | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | *Gel results: | ||
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Revision as of 10:14, 8 September 2009
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Week from August 31st, to September 6th, 2009
Previous Week
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Next Week
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August, 31st
- We inoculated:
- 10 colonies from B5new2 plate in 4 ml of LB + Kan for screening;
- B8-5 (8 ul from glycerol stock) in 4 ml of LB + Kan (for purification process);
- We sent A15-2 purified DNA (stored at -20°C) to BMR Genomics for sequencing.
Experiment with Tecan F200
- Description
- Purpose:
- Materials & Methods
- Protocol
- Results
September, 1st
- We received sequencing results for:
- Glycerol stocks for the 10 B5new2 grown cultures.
- Miniprep for B5new2 (10 samples) and for B8-5.
- Digestion for:
- B5new2 (10 samples) for screening (E-P cut);
- B8-5 for purification from gel (E-P cut);
- R0011 (stored at -20°C) for A17new ligation (S-P cut)
- E0240 (stored at -20°C) for A17new ligation (X-P cut)
- Medium-size gel for B5new2(E-P); small-size gel for B8-5(E-P), R0011(S-P) and E0240(X-P).
- Gel results:
September, 2nd
September, 3rd
September, 4th<
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Previous Week
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Next Week
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