EPF-Lausanne/3 September 2009

(Difference between revisions)

Latest revision as of 14:32, 8 September 2009

There is clones on every plate!

→ colony PCR on all clones with the Taq Platinium protocol

All media have worked! (except for the SC) They have different colors depending on media and clone after an overnight culture.

We tried the following media, with and without Trp :

LB

M9/min + thiamine

M9/min + AA + glycerol

M9/min + thiamine

The gel showed :

RO1: it works fine, all our constructs have the TrpO

RO2: #6 and #7 do not have the construct (we took the wrong clones), while #8 seems to have the construct!

Basile, Mélanie, Caroline

@@ Line 28: / Line 28: @@
 * '''Results of the RO1:'''
 There is clones on every plate!
-: → colony PCR on all clones with th eTaq Platinium protocol
+: → colony PCR on all clones with the Taq Platinium protocol
 <br>
 * '''Results from the media try:'''
-<br>All media have worked! (except for the SC) They have different colors depending on media and clone after an overnight culture.
+All media have worked! (except for the SC) They have different colors depending on media and clone after an overnight culture.
 {| class="wikitable" style="text-align:center; width:80%;"
@@ Line 50: / Line 50: @@
 |LB
 |-
-! scope=row | 9/min + AA + thiamine
+! scope=row | M9/min + AA + thiamine
 |rose
 |white
 |white
 |-
-! scope=row | 9/min + thiamine
+! scope=row | M9/min + thiamine
 |rose
 |rose
@@ Line 67: / Line 67: @@
+* '''Culture for characterization'''
+We tried the following media, with and without Trp :
+: LB
+: M9/min + thiamine
+: M9/min + AA + glycerol
+: M9/min + thiamine
+The gel showed :
+: '''RO1''': it works fine, all our constructs have the TrpO
+: '''RO2''': #6 and #7 do not have the construct (we took the wrong clones), while #8 seems to have the construct!
 ==People in the lab==