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EPF-Lausanne/3 September 2009
From 2009.igem.org
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* '''Results of the RO1:''' | * '''Results of the RO1:''' | ||
There is clones on every plate! | There is clones on every plate! | ||
| - | : → colony PCR on all clones with | + | : → colony PCR on all clones with the Taq Platinium protocol |
<br> | <br> | ||
* '''Results from the media try:''' | * '''Results from the media try:''' | ||
| Line 68: | Line 68: | ||
* '''Culture for characterization''' | * '''Culture for characterization''' | ||
| - | We tried the following media, with and | + | We tried the following media, with and without Trp : |
: LB | : LB | ||
: M9/min + thiamine | : M9/min + thiamine | ||
Latest revision as of 14:32, 8 September 2009
Contents |
Wet Lab
- Results of the RO1:
There is clones on every plate!
- → colony PCR on all clones with the Taq Platinium protocol
- Results from the media try:
All media have worked! (except for the SC) They have different colors depending on media and clone after an overnight culture.
| RO2 #6 | RO2 #7 | RO2 #8 | |
|---|---|---|---|
| M9/min + AA + glycerol | white | white | white |
| LB | LB + slight red | LB + red | LB |
| M9/min + AA + thiamine | rose | white | white |
| M9/min + thiamine | rose | rose | white |
| SC | NA | NA | NA |
- Culture for characterization
We tried the following media, with and without Trp :
- LB
- M9/min + thiamine
- M9/min + AA + glycerol
- M9/min + thiamine
The gel showed :
- RO1: it works fine, all our constructs have the TrpO
- RO2: #6 and #7 do not have the construct (we took the wrong clones), while #8 seems to have the construct!
People in the lab
Basile, Mélanie, Caroline
