Template:Team:KULeuven/7 September 2009/BlueLightReceptor

From 2009.igem.org

(Difference between revisions)
(New page: # Restriction digest with EcoRI and PstI on {{kulpart|BBa_E0240}} and the purified pcr product of {{kulpart|BBa_K238013}} in order to get our promotor on intself in the pSB1A2 standard vec...)
 
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# Restriction digest with EcoRI and PstI on {{kulpart|BBa_E0240}} and the purified pcr product of {{kulpart|BBa_K238013}} in order to get our promotor on intself in the pSB1A2 standard vector.  
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# Restriction digest with EcoRI and PstI on {{kulpart|BBa_E0240}} and the purified pcr product of {{kulpart|BBa_K238013}} in order to get our promotor on itself in the {{kulpart|pSB1A2}} standard vector.  
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# gel electroforesis and extraction of this RD. The concentration of this extraction was too low so no ligation was performed with these samples.
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# Gel electroforesis and extraction of this RD. The concentration of this extraction was too low so no ligation was performed with these samples.
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# extra pcr was done on {{kulpart|BBa_K238013}} with primers 2260 and 2261.   
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# Extra pcr was done on {{kulpart|BBa_K238013}} with primers 2260 and 2261.   
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# liquid cultures of LigX and of {{kulpart|BBa_J23101}} were made from the 4x plates.
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# Liquid cultures of LigX and of {{kulpart|BBa_J23101}} were made from the 4x plates.

Latest revision as of 08:31, 14 September 2009

  1. Restriction digest with EcoRI and PstI on and the purified pcr product of in order to get our promotor on itself in the standard vector.
  2. Gel electroforesis and extraction of this RD. The concentration of this extraction was too low so no ligation was performed with these samples.
  3. Extra pcr was done on with primers 2260 and 2261.
  4. Liquid cultures of LigX and of were made from the 4x plates.
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