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Template:Team:KULeuven/5 August 2009/VanillinProduction
From 2009.igem.org
(Difference between revisions)
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Miniprep of pairs: | Miniprep of pairs: | ||
| - | * RBS + | + | * RBS + ''sam5'' {{kulpart|BBa_K238007}} conc: 101,3 ng/ul |
| - | * RBS+ | + | * RBS + ''sam8'' {{kulpart|BBa_I742106}} conc: 95.7 ng/ul |
| - | * | + | * ''sam8'' {{kulpart|BBa_I742142}} conc: 81,8 ng/ul |
| - | * | + | * ''COMT'' {{kulpart|BBa_I742107}}, conc: 122,9 ng/ul |
Restriction digestion<br> | Restriction digestion<br> | ||
| - | A1: ''' | + | A1: '''''sam8''''', EcoRI en SpeI <br> |
| - | A2: ''' | + | A2: '''''sam5''''', EcoRI en XbaI<br> |
<br> | <br> | ||
B1: '''RBS''', EcoRI en SpeI<br> | B1: '''RBS''', EcoRI en SpeI<br> | ||
| - | B2: ''' | + | B2: '''''COMT''''', EcoRI en XbaI<br> |
<br> | <br> | ||
| - | C1: ''' | + | C1: '''''fcs''''', EcoRI en SpeI <br> |
| - | C2: ''' | + | C2: '''''ech''''', EcoRI en XbaI<br> |
The mixture was incubated in 37°C for 1h30... | The mixture was incubated in 37°C for 1h30... | ||
| - | ...followed by | + | ...followed by gel electrophoresis of the digested parts |
Latest revision as of 09:49, 14 September 2009
Miniprep of pairs:
- RBS + sam5 conc: 101,3 ng/ul
- RBS + sam8 conc: 95.7 ng/ul
- sam8 conc: 81,8 ng/ul
- COMT , conc: 122,9 ng/ul
Restriction digestion
A1: sam8, EcoRI en SpeI
A2: sam5, EcoRI en XbaI
B1: RBS, EcoRI en SpeI
B2: COMT, EcoRI en XbaI
C1: fcs, EcoRI en SpeI
C2: ech, EcoRI en XbaI
The mixture was incubated in 37°C for 1h30...
...followed by gel electrophoresis of the digested parts
