Template:Team:KULeuven/24 August 2009/BlueLightReceptor

From 2009.igem.org

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Latest revision as of 12:25, 14 September 2009

Plates with LigA were put under blue light. The LEDs were put on their max capacity.
Restriction digest with
- tubes (1,3,5,7,9) of LigA (BLP + ) cut with EcoRI en PstI
- promotor cut with EcoRI en SpeI (4x)
Gel electrophoresis with the RD of LigA and followed by a gel extraction:
- Note: tube 5 of LigA was loaded poorly on the gel and could not be used.
- Note: the samples of promotor were barely visible. Only 2 of the 4 samples were recovered by extraction.

Part	concentration (ng/μl)	260/280 λ
LigA 1	7,9	2,16
LigA 3	10,8	1,88
LigA 7	4,8	2,49
LigA 9	8,0	3,02
(A)	20,8	1,70
(B)	12,8	2,57

4. Enting of liquid cultures with kanamycin and

@@ Line 1: / Line 1: @@
-# restrictie digest with
+# Plates with LigA were put under blue light. The LEDs were put on their max capacity.
-#*tubes (1,3,5,7,9) of ligA (BLP + E0240) cut with EcoRI en PstI
+# Restriction digest with
-#*promotor J23101 cut with EcoRI en SpeI (4x)
+#*tubes (1,3,5,7,9) of LigA (BLP + {{kulpart|BBa_E0240}}) cut with EcoRI en PstI
-#gel electroforese with the RD of ligA and J23101 followed by a gel extraction:
+#*promotor {{kulpart|BBa_J23101}} cut with EcoRI en SpeI (4x)
-#* note: tube 5 of ligA was loaded poorly on the gel and could not be used.
+#Gel electrophoresis with the RD of LigA and {{kulpart|BBa_J23101}} followed by a gel extraction:
-#* note: the samples of promotor J23101 were barly visible. only 2 of the 4 samples were recoverd by extraction.
+#* Note: tube 5 of LigA was loaded poorly on the gel and could not be used.
+#* Note: the samples of promotor {{kulpart|BBa_J23101}} were barely visible. Only 2 of the 4 samples were recovered by extraction.
 {| border ="1" align="center"
-!| Part || concentration (ng/&mu;l) || 260/280 &lambda; || 260/230 &lambda;
+!| Part || concentration (ng/&mu;l) || 260/280 &lambda; ||
 |- align="center"
-| ligA 1 ||7,9 || 2,16 ||
+| LigA 1 ||7,9 || 2,16 ||
 |- align="center"
-| ligA 3 ||10,8 || 1,88 ||
+| LigA 3 ||10,8 || 1,88 ||
 |- align="center"
-| ligA 7 ||4,8 || 2,49 ||
+| LigA 7 ||4,8 || 2,49 ||
 |- align="center"
-| ligA 9 ||8,0 || 3,02 ||
+| LigA 9 ||8,0 || 3,02 ||
 |- align="center"
 | {{kulpart|BBa_J23101}} (A) ||20,8|| 1,70 ||
 |- align="center"
 | {{kulpart|BBa_J23101}} (B) ||12,8|| 2,57 ||
+|}
+. Enting of liquid cultures with kanamycin and {{kulpart|pSB3K3}}