Team:Warsaw/Calendar-Main/9 September 2009
From 2009.igem.org
(Difference between revisions)
(One intermediate revision not shown) | |||
Line 23: | Line 23: | ||
*Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described [http://aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf here] | *Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described [http://aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf here] | ||
- | + | <html> | |
+ | <h3><div style="text-align: center;">Inverting PLacI regulated parts of the switch and assembling the switch on a single plasmid</div><h3> | ||
+ | <h4>Ania</h4> | ||
+ | <br/> | ||
+ | <p>Tasks:</p> | ||
+ | <ul> | ||
+ | <li> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <br/> | ||
+ | </html> | ||
<!-- do not remove this! --> | <!-- do not remove this! --> | ||
{{WarNotebookEnd}} | {{WarNotebookEnd}} |
Latest revision as of 20:38, 17 September 2009
Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome
Jarek
Tasks:
- Transformation of competent E.coli strain TopF' with ligation.
- Plating ligation on LB+X-Gal+IPTG+Amp plates and leaving for incubation in 37C degree.
Assembly of fusion proteins
Marcin
Task 1 (done by Kuba):
- Prepare the bacterial cultures for isolation of following construct:
- mitochondrial signal peptide from Cox CDS on pKSII plasmid
Task 2
- isolation of previously described construct
Methods:
- Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described [http://aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf here]
Inverting PLacI regulated parts of the switch and assembling the switch on a single plasmid
Ania
Ania
Tasks:
|
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|