Virginia Commonwealth/6 June 2009
From 2009.igem.org
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| .26 || .018 || 1.44 || 52 || 1300 || 25 || | | .26 || .018 || 1.44 || 52 || 1300 || 25 || | ||
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| .085 || .050 || 1.700 || 17 || 969 || 57 || | | .085 || .050 || 1.700 || 17 || 969 || 57 || | ||
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| .063 || .038 || 1.658 || 12.6 || 453 || 36 || | | .063 || .038 || 1.658 || 12.6 || 453 || 36 || | ||
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- | ! | + | ! pSB4C5 |
| .101 || .059 || 1.712 || 20.2 || 767 || 38 || | | .101 || .059 || 1.712 || 20.2 || 767 || 38 || | ||
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Revision as of 16:30, 10 June 2009
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Contents |
Saturday 06 June 2009
Results
- Note that undergraduates need key card access to the building.
- All samples from yesterday's cultivation grew well.
Tasks
- Harvested cells from cultivation
- Purified plasmids using the mini-prep kit
- Froze stock of cells carrying plasmids in box C-15
Wetlab
- Mini-prep (plasmid purification) tutorial
- Data from plasmid purification
Part No. | A260 (nm) | A280 (nm) | A260/A280 | Concentration (microgram/microliter) | Amount (microgram) | Volume (microliter) | |
---|---|---|---|---|---|---|---|
J13002 | .26 | .018 | 1.44 | 52 | 1300 | 25 | |
pSB1C3 | .085 | .050 | 1.700 | 17 | 969 | 57 | |
R0040 | .025 | .017 | 1.471 | 5 | 200 | 40 | |
E1010 | .063 | .038 | 1.658 | 12.6 | 453 | 36 | |
pSB4C5 | .101 | .059 | 1.712 | 20.2 | 767 | 38 | |
E0840 | .084 | .049 | 1.714 | 16.8 | 688 | 41 |
- Bussingkm 23:10, 6 June 2009 (UTC)