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-	*2	+	*Problems:
		+	**Part J23106 was taken from well 18D, when it actually is located in well 18O.
		+	**Part Q04511 was actually taken from well 18D
		+	**Part R0011 was plated twice. The plate with the most colonies was used for growth overnight.
	[[User:MandM|MandM]] 22:52, 11 June 2009 (UTC)		[[User:MandM|MandM]] 22:52, 11 June 2009 (UTC)

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Revision as of 23:05, 11 June 2009

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July M T W T F S S     [http://2009.igem.org/Virginia_Commonwealth/1_July_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_July_2009 2] [http://2009.igem.org/Virginia_Commonwealth/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_July_2009&action=edit 5] [http://2009.igem.org/Virginia_Commonwealth/6_July_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_July_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_July_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_July_2009 9] [http://2009.igem.org/Virginia_Commonwealth/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_July_2009&action=edit 12] [http://2009.igem.org/Virginia_Commonwealth/13_July_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_July_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_July_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_July_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_July_2009&action=edit 19] [http://2009.igem.org/Virginia_Commonwealth/20_July_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_July_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_July_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_July_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/26_July_2009&action=edit 26] [http://2009.igem.org/Virginia_Commonwealth/27_July_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_July_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_July_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_July_2009 30] [http://2009.igem.org/Virginia_Commonwealth/31_July_2009 31]

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October M T W T F S S       [http://2009.igem.org/Virginia_Commonwealth/1_October_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_October_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_October_2009&action=edit 4] [http://2009.igem.org/Virginia_Commonwealth/5_October_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_October_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_October_2009&action=edit 11] [http://2009.igem.org/Virginia_Commonwealth/12_October_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_October_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_October_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_October_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]

Contents 1 Thursday 11 June 2009 1.1 Results 1.2 Tasks 1.2.1 Wetlab

Thursday 11 June 2009

Results

• Note these were plated Wednesday evening with two groups splitting the work.

• Group 1: Craig, Clay, Afton plates
• Observations on the Controls

Control	Plate Type	Observation
NEB10β (+)	LB	lawn
NEB10β (-)	Cm	no growth
NEB10β (-)	Amp	no growth
NEB10β (-)	Kan	3 colonies	The possibilities for this are a non-homogeneous medium with very small sections capable of sustaining life or there was degradation of the Kan-selective medium.

• Observations on the Colonies

Part No.	Plate Type	Colonies	Other Observations
pSB 3K3	Kan	10s of colonies	n/a
E0240	Amp	10s of colonies
J23113	Amp	10s of colonies	n/a
J23116	Amp	100s of colonies	n/a
J23101	Amp	about 9 colonies	n/a
R0040	Amp	10s of colonies	n/a

• Group 2: Adam, Kevin and Maria Plates
• Observations on the Colonies

Part No.	Plate Type	Colonies	Other Observations
R0011	Amp	about 9 colonies	n/a
J23100	Amp	10s of colonies	n/a
J23103	Amp	10s of colonies	The colonies are pinkish in color because the rfp is being expressed.
J23104	Amp	10s of colonies	n/a
J23105	Amp	100s of colonies	n/a
J23106	Amp	about 9 colonies	n/a
J23107	Amp	10s of colonies	n/a

• Problems:
  • Part J23106 was taken from well 18D, when it actually is located in well 18O.
  • Part Q04511 was actually taken from well 18D
  • Part R0011 was plated twice. The plate with the most colonies was used for growth overnight.

MandM 22:52, 11 June 2009 (UTC)

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Tasks

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• Literature Discussion
• Discussion of iGEM ideas

Wetlab

• Picking Transformed Colonies to be grown overnight for Mini-preps (5mL) and two Maxi-preps(125mL).

MandM 22:55, 11 June 2009 (UTC)

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