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Team:Cambridge/Notebook/Week10
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Following the ligation, the constructs 50, 51, 52 ligated to B0015 were transformed into the arabinose strain. As a control, the products of the digest and gel extraction including all of the parts pre-ligation were also transformed into the same strain in order to check whether the ligation had worked. | Following the ligation, the constructs 50, 51, 52 ligated to B0015 were transformed into the arabinose strain. As a control, the products of the digest and gel extraction including all of the parts pre-ligation were also transformed into the same strain in order to check whether the ligation had worked. | ||
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| + | The products of the digest and extraction are to be found in Box 1, labelled 50d, 51d, 52d, B0015d 1.5 and B0015d 2.0 (as there were two bands for this part following the digest, both were extracted). | ||
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Revision as of 14:39, 24 September 2009
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
Week 10
Monday
Wet Work
Threshold Devices
Confirmed successful transfer of 40 (pBad followed by GFP) into the low copy plasmid pSB3K3. Transformed 40 in pSB3K3 into arabinose strain.
Confirmed successful construction of pBad + activator (50, 51, and 52) + B0015.
Transformation of TOP10 with activator constructs 50, 51, 52 and the part B0015 in preparation for ligation to pigment parts.
Tuesday
Dry Work
Wet Work
Transformants from yesterday put into overnight culture.
Wednesday
Wet Work
Activator constructs 50, 51, 52 and the part B0015 were mini-prepped from the overnight culture and run on a gel for extraction and purification. Ligations between each of the activators and B0015 were then carried out.
Thursday
Wet Work
Following the ligation, the constructs 50, 51, 52 ligated to B0015 were transformed into the arabinose strain. As a control, the products of the digest and gel extraction including all of the parts pre-ligation were also transformed into the same strain in order to check whether the ligation had worked.
The products of the digest and extraction are to be found in Box 1, labelled 50d, 51d, 52d, B0015d 1.5 and B0015d 2.0 (as there were two bands for this part following the digest, both were extracted).
Friday
