Team:Imperial College London/Wetlab/Protocols/PromoterCharacterisation

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| <b>2.Auto-Induction</b>
 
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| [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |IPTG ]]
 
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* Characterise Lac promoter by varying concentrations of IPTG
 
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* Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell
 
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Revision as of 10:56, 30 September 2009

Calibration protocols (CA)

Section Assay Overview and Aims
2.Auto-Induction IPTG
  • Characterise Lac promoter by varying concentrations of IPTG
  • Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell

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