"
Team:Imperial College London/Wetlab/Protocols/PromoterCharacterisation
From 2009.igem.org
(Difference between revisions)
(New page: {| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3" ! Section ! Assay ! Overview and Aims |- style="color:#333; background-color:#CCCCFF;" cellpadd...) |
(→Calibration protocols (CA)) |
||
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{Imperial/09/TemplateTop}} | ||
| + | =Promoter Characterisation (PC)= | ||
{| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3" | {| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3" | ||
! Section | ! Section | ||
! Assay | ! Assay | ||
! Overview and Aims | ! Overview and Aims | ||
| - | |||
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| + | | <b>Promoter Characterisation</b> | ||
| + | | [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |IPTG ]] | ||
| + | | | ||
| + | * Characterise Lac promoter by varying concentrations of IPTG | ||
| + | * Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell | ||
| + | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
| + | |||
| + | |} | ||
| + | |||
| + | {{Imperial/09/TemplateBottom}} | ||
Latest revision as of 10:56, 30 September 2009
Promoter Characterisation (PC)
| Section | Assay | Overview and Aims |
|---|---|---|
| Promoter Characterisation | IPTG |
|
