Team:Imperial College London/Wetlab/Protocols/PromoterCharacterisation

From 2009.igem.org

(Difference between revisions)
(Calibration protocols (CA))
 
(2 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Imperial/09/TemplateTop}}
 +
=Promoter Characterisation (PC)=
{| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3"
{| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3"
! Section
! Section
! Assay
! Assay
! Overview and Aims
! Overview and Aims
-
 
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
-
| <b>2.Auto-Induction</b>
+
| <b>Promoter Characterisation</b>
| [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |IPTG ]]
| [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |IPTG ]]
|  
|  
Line 12: Line 13:
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
-
|
 
|}
|}
 +
 +
{{Imperial/09/TemplateBottom}}

Latest revision as of 10:56, 30 September 2009

Promoter Characterisation (PC)

Section Assay Overview and Aims
Promoter Characterisation IPTG
  • Characterise Lac promoter by varying concentrations of IPTG
  • Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell

Mr. Gene   Geneart   Clontech   Giant Microbes