Latest revision as of 10:56, 30 September 2009

Promoter Characterisation (PC)

Section	Assay	Overview and Aims
Promoter Characterisation	IPTG	Characterise Lac promoter by varying concentrations of IPTG Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell

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-=Calibration protocols (CA)=
+=Promoter Characterisation (PC)=
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 ! Section
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 ! Overview and Aims
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-| <b>2.Auto-Induction</b>
+| <b>Promoter Characterisation</b>
 | [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |IPTG ]]
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-|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
-| <b>2.Auto-Induction</b>
-| [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |IPTG ]]
-|
-*	Characterise Lac promoter by varying concentrations of IPTG
-*	Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell
-|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"