EPF-Lausanne/9 September 2009

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(Difference between revisions)
(Characterization)
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RBS was our negative control. We can see that there is no RFP fluorescence (because of course RBS has no RFP gene).
RBS was our negative control. We can see that there is no RFP fluorescence (because of course RBS has no RFP gene).
-
For LacI-RFP + IPTG :  
+
For LacI-RFP 1 + IPTG :  
[[Image:IPTG.jpg|center|LacI-RFP + IPTG plot]]
[[Image:IPTG.jpg|center|LacI-RFP + IPTG plot]]
 +
LacI-RFP 2 + IPTG show the same tendancy.
 +
RO1#1 + 0.5 TRP :
 +
[[Image:RO1105TRP.jpg|center|RO1#1 +0.5 TRP]]
 +
RO1#2 + 0.5 TRP shows the same tendancy.
==People in the lab==
==People in the lab==

Revision as of 10:52, 2 October 2009

Contents

9 September 2009





Wet Lab

Characterization



With Atc Without Atc
With Trp
1/2 Trp + 1/2 Atc
1 Trp + 1/2 Atc
1/2 Trp + 1 Atc
1 Trp + 1 Atc
1/2 Trp
1 Trp
3/2 Trp
Without Trp 1/2 Atc
1 Atc
3/2 Atc
Without Atc nor Trp


Results of the characterization

For the RBS :

RBS plot

RBS was our negative control. We can see that there is no RFP fluorescence (because of course RBS has no RFP gene).

For LacI-RFP 1 + IPTG :

LacI-RFP + IPTG plot

LacI-RFP 2 + IPTG show the same tendancy.

RO1#1 + 0.5 TRP :

RO1#1 +0.5 TRP

RO1#2 + 0.5 TRP shows the same tendancy.

People in the lab

Mélanie, Caroline, Basile, Nicolas