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EPF-Lausanne/9 September 2009
From 2009.igem.org
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Clone #2 has the same behaviour. | Clone #2 has the same behaviour. | ||
| - | RO1#3 clones doesn't follow these tendancy. It actually seems that this clone isn't working at all. It is possible a spontaneous mutation occured in some place... On all different conditions, RO1#3 clones show a flat curve, like RBS. It doesn't express RFP which means either our construct wasn't inserted, either there has been a mutation in it. | + | RO1#3 clones doesn't follow these tendancy. It actually seems that this clone isn't working at all. It is possible a spontaneous mutation occured in some place... On all different conditions, RO1#3 clones show a flat curve, like RBS. It doesn't express RFP which means either our construct wasn't inserted, either there has been a mutation in it. For example : |
| - | + | [[Image:RO131TRP.jpg|center|RO1#3 + 1 TRP]] | |
'''For Read Out 2''' | '''For Read Out 2''' | ||
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[[Image:RO2115ATC.jpg|center|RO2#1 + 1.5 ATC]] | [[Image:RO2115ATC.jpg|center|RO2#1 + 1.5 ATC]] | ||
| + | |||
| + | Clone RO2#2 is not working, it has always a flat curve like this : | ||
| + | [[Image:RO2205ATC.jpg|center|RO2#2 + 0.5 ATC]] | ||
==People in the lab== | ==People in the lab== | ||
Revision as of 11:22, 2 October 2009
Wet Lab
Characterization
| With Atc | Without Atc | |||
|---|---|---|---|---|
| With Trp |
| 1/2 Trp 1 Trp 3/2 Trp | ||
| Without Trp | 1/2 Atc 1 Atc 3/2 Atc | Without Atc nor Trp |
Results of the characterization
For the RBS :
RBS was our negative control. We can see that there is no RFP fluorescence (because of course RBS has no RFP gene).
For LacI-RFP 1 + IPTG :
LacI-RFP 2 + IPTG show the same tendancy.
For Read Out 1
RO1#1 + 0.5 TRP :
RO1#2 + 0.5 TRP shows the same tendancy.
RO1#1 + 1 TRP :
Again, RO1#2 clone has the same behaviour.
RO1#1 without TRP:
Clone #2 has the same behaviour.
RO1#3 clones doesn't follow these tendancy. It actually seems that this clone isn't working at all. It is possible a spontaneous mutation occured in some place... On all different conditions, RO1#3 clones show a flat curve, like RBS. It doesn't express RFP which means either our construct wasn't inserted, either there has been a mutation in it. For example :
For Read Out 2
Clone RO2#2 is not working, it has always a flat curve like this :
People in the lab
Mélanie, Caroline, Basile, Nicolas
