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EPF-Lausanne/24 September 2009
From 2009.igem.org
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==Wet Lab== | ==Wet Lab== | ||
===Transformation in Trp K.O.=== | ===Transformation in Trp K.O.=== | ||
| - | The 3 newly competent strains (JW4356-2, JRG 1046, JRG 465) will be transformed | + | The 3 newly competent strains (JW4356-2, JRG 1046, JRG 465) will be transformed with : |
| + | |||
| + | - RO2#4 + BB1 (+Amp, Kana) -> double transformation. | ||
| + | |||
| + | - RO2#4 (+Kana) | ||
| + | |||
| + | - RO1#1 (+Kana) | ||
| + | |||
| + | - RO1#1 + BB1 (+Amp, Kana) -> double transformation. | ||
| + | |||
| + | Note : the 3 control (1 ug/ul DNA, 100 pg/ul DNA, 10 pg/ul DNA -> 10ul for each transf) + KANA. We will count the nb of clones the next morning on the plates to determine transformation efficiency. | ||
==People in the lab== | ==People in the lab== | ||
Revision as of 14:09, 4 October 2009
Wet Lab
Transformation in Trp K.O.
The 3 newly competent strains (JW4356-2, JRG 1046, JRG 465) will be transformed with :
- RO2#4 + BB1 (+Amp, Kana) -> double transformation.
- RO2#4 (+Kana)
- RO1#1 (+Kana)
- RO1#1 + BB1 (+Amp, Kana) -> double transformation.
Note : the 3 control (1 ug/ul DNA, 100 pg/ul DNA, 10 pg/ul DNA -> 10ul for each transf) + KANA. We will count the nb of clones the next morning on the plates to determine transformation efficiency.
People in the lab
