Revision as of 14:52, 5 October 2009

Lab Work - 14/09/09

Today's lab session will continue straight on from yesterday's session and fulfil the following objectives:

Run products of PCR reaction involving the arsR BioBrick (BBa_J33206 in pSB1A2 missing promoter) and cadA promoter region
- If successful clean up product, run on agarose gel through electrophoresis, excise band and carry out gel extraction.
- Cut cleaned and excised fragment with BamHI and NheI
- Carry out ligation (possibly an overnight ligation)
Cut pMUTIN4 and cotC (i.e. PCR product 3) with BamHI and HindIII
- Ligate the fragments (possibly overnight)

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 __NOTOC__
 =Lab Work - 14/09/09=
+==Metal Sensing team==
+===Introduction===
+===Practical Outline===
+Today's lab session will continue straight on from yesterday's session and fulfil the following objectives:
+<br>
+* Run products of PCR reaction involving the arsR BioBrick (BBa_J33206 in pSB1A2 missing promoter) and cadA promoter region
+** If successful clean up product, run on agarose gel through electrophoresis, excise band and carry out gel extraction.
+** Cut cleaned and excised fragment with BamHI and NheI
+** Carry out ligation (possibly an overnight ligation)
+* Cut pMUTIN4 and cotC (i.e. PCR product 3) with BamHI and HindIII
+** Ligate the fragments (possibly overnight)
+<br>
+===Procedure===
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