Team:Newcastle/Labwork/17 September 2009

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=Lab Work - 17/09/09=
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=Formal Lab Session - 17th September 2009=
=Stochastic switch team=
=Stochastic switch team=
Today we ran the minipreps for sac and ara transformed cells however- perhaps due to poor miniprep procedure- the sac test digest yeilded poor results, however the ara transformants miniprep gave a ~200bp band along with the backbone in 8/12 lanes: [[Image:Team NewcastleAra 170909.png]]
Today we ran the minipreps for sac and ara transformed cells however- perhaps due to poor miniprep procedure- the sac test digest yeilded poor results, however the ara transformants miniprep gave a ~200bp band along with the backbone in 8/12 lanes: [[Image:Team NewcastleAra 170909.png]]

Revision as of 16:15, 6 October 2009

Team Newcastle 2009 iGEM ProbationaryP-Sign.PNG

Formal Lab Session - 17th September 2009

Stochastic switch team

Today we ran the minipreps for sac and ara transformed cells however- perhaps due to poor miniprep procedure- the sac test digest yeilded poor results, however the ara transformants miniprep gave a ~200bp band along with the backbone in 8/12 lanes: Team NewcastleAra 170909.png

Midi prep cultures were set up using cultures corresponding to lanes 1 and 3 for ara.

To test whether the poor sac result was due to the miniprep more miniprep cultures were set up to do tomorrow.

Today also we did fragment preps for Goksels sspb the ara fragment, and a cwlJ fragment for Hanny: Team NewcFragpreps1.png



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