Team:Newcastle/Labwork/15 September 2009
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Yesterday we did midipreps of Goksels successful sspb plasmid: this was cut with XbaI and PstI ready for ligation into the ara brick (once it's ligated!). | Yesterday we did midipreps of Goksels successful sspb plasmid: this was cut with XbaI and PstI ready for ligation into the ara brick (once it's ligated!). | ||
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Revision as of 13:03, 7 October 2009
Formal Lab Session - 15th September 2009
Stochastic Switch Team
Today we set up another restriction disest of the KinA pMKRQ synthesised brick using NheI and EcoRI we ran this on an 0.8% gel however we think that this may be too dilute and that I why we are not seeing the two bands ogf the digest which are a similar size.
We also set up another PCR of the ara fragment as a back up plan for ligation, in case the last ligation did not work as well as we had planned.
We also mini prepped and digested the sac transformations again, and we found that of the 6 samples we had, a few of them had worked!
Just to confirm however we set up 12 more miniprep cultures again for both sac and ara.
Yesterday we did midipreps of Goksels successful sspb plasmid: this was cut with XbaI and PstI ready for ligation into the ara brick (once it's ligated!).
Metal Sensing Team
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]