August/30 August 2009

From 2009.igem.org

(Difference between revisions)

Latest revision as of 08:22, 12 October 2009

1. ligation rapid check

UP   : 13-A,B,C  ,  14-A,B,C  , 19-A,B,C  , vector ,ladder
DOWN : 12-A,B,C  ,  15-A,B,C  , 16-A,B,C  , vector , ladder

No.13 (RBS+rhlI),K204013

13-A,C : OK

No.14 (RBS+LasI),K204014

14 -A,C : OK

No.19 (RBS+RFP(orange?)),K204019

19-A,B : OK

No.12 (RBS+cinR+terminater),K204012

12-B  : OK ?

No.15 (RBS+cinI+terminater), K204015

15-A,B,C : OK?

No.16 (RBS+LuxI+terminater) , K204016

16-A,B,C ok?

2.digestion and ligation

digestion with restriction enzyme

K204020

Vector		Insert
p(tetR)([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 1-6I])	5	YFP([http://partsregistry.org/wiki/index.php?title=Part:BBa_E0430 1-8I])	5
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2 Buffer	2	No.2	2
dH₂O	11	dH₂O	4
total	20uL	total	20uL

K204021

Vector		Insert
p(tetR)([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 1-6I])	5	CFP([http://partsregistry.org/wiki/index.php?title=Part:BBa_E0420 1-8K])	5
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2 Buffer	2	No.2	2
dH₂O	11	dH₂O	11
total	20uL	total	20uL

3.12-B , 13-A , 14-A , 15-A , 16-A , 19-A were inoculated into growth culture solution.
Miniprep should be conducted on the resulting cell cultures tomorrow.

back to NOTES

@@ Line 1: / Line 1: @@
 . '''ligation rapid check'''<br>
+[[Image:Osaka090830.jpg]]
+ UP   : 13-A,B,C  ,  14-A,B,C  , 19-A,B,C  , vector ,ladder
+ DOWN : 12-A,B,C  ,  15-A,B,C  , 16-A,B,C  , vector , ladder
 No.13 (RBS+rhlI),K204013
@@ Line 28: / Line 33: @@
 <table border="1" Frame="box">
 <tr><th colspan="2">Vector</th><th colspan="2">Insert</th></tr>
-<tr><td>p(tetR)(1-6I)</td><td>5</td><td>YFP(1-8I)</td><td>5</td></tr>
+<tr><td>p(tetR)([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 1-6I])</td><td>5</td><td>YFP([http://partsregistry.org/wiki/index.php?title=Part:BBa_E0430 1-8I])</td><td>5</td></tr>
 <tr><td>SpeI</td><td>1</td><td>XbaI</td><td>1</td><tr>
 <tr><td>PstI</td><td>1</td><td>PstI</td><td>1</td><tr>
@@ Line 40: / Line 45: @@
 <table border="1" Frame="box">
 <tr><th colspan="2">Vector</th><th colspan="2">Insert</th></tr>
-<tr><td>p(tetR)(1-6I)</td><td>5</td><td>CFP(1-8K)</td><td>5</td></tr>
+<tr><td>p(tetR)([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 1-6I])</td><td>5</td><td>CFP([http://partsregistry.org/wiki/index.php?title=Part:BBa_E0420 1-8K])</td><td>5</td></tr>
 <tr><td>SpeI</td><td>1</td><td>XbaI</td><td>1</td><tr>
 <tr><td>PstI</td><td>1</td><td>PstI</td><td>1</td><tr>
@@ Line 51: / Line 56: @@
 .'''12-B , 13-A , 14-A , 15-A , 16-A , 19-A ''' were inoculated into growth culture solution. <br>
 Miniprep should be conducted on the resulting cell cultures tomorrow.
+[https://2009.igem.org/Team:Osaka/NOTES back to NOTES]