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Team:Groningen/Modelling/Characterization
From 2009.igem.org
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<script type="text/javascript" src="/Team:Groningen/Modelling/Model.js?action=raw"></script> | <script type="text/javascript" src="/Team:Groningen/Modelling/Model.js?action=raw"></script> | ||
<script type="text/javascript"> | <script type="text/javascript"> | ||
| - | var experiments = {Meng2004: | + | var experiments = {/*Meng2004: |
{constants:{Vc:0.0073,Vs:(1.1-0.0073),beta4:0,pro:0,ars2T:0},AsT:10e-6, | {constants:{Vc:0.0073,Vs:(1.1-0.0073),beta4:0,pro:0,ars2T:0},AsT:10e-6, | ||
data:{AsinT:[101.917808219178e-6,394.520547945205e-6,723.287671232877e-6, | data:{AsinT:[101.917808219178e-6,394.520547945205e-6,723.287671232877e-6, | ||
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0.361791516e-6,0.332907991e-6,0.320748614e-6], | 0.361791516e-6,0.332907991e-6,0.320748614e-6], | ||
time:[60,300,600,1200,1800,3600] | time:[60,300,600,1200,1800,3600] | ||
| - | /*[1.127*60,4.993*60,9.986*60,20.159*60,30.181*60,60.035*60]*/}}, | + | *//*[1.127*60,4.993*60,9.986*60,20.159*60,30.181*60,60.035*60]*//*}}, |
| - | /*Kostal2004: // fig 2C | + | *//*Kostal2004: // fig 2C |
{constants:{Vc:0.006666667,Vs:(1-0.006666667),pro:0,ars2T:0},AsT:10e-6, | {constants:{Vc:0.006666667,Vs:(1-0.006666667),pro:0,ars2T:0},AsT:10e-6, | ||
data:{AsinT:[159.39e-6,491.7e-6,679.8e-6,742.5], | data:{AsinT:[159.39e-6,491.7e-6,679.8e-6,742.5], | ||
| - | time:[44928,83520,132192,171036]}},*/ | + | time:[44928,83520,132192,171036]}},*//* |
Kostal2004fig3A: // fig 3A | Kostal2004fig3A: // fig 3A | ||
{constants:{Vc:0.006666667,Vs:(1-0.006666667),pro:0,ars2T:0,proK:16.605e-9},time:Infinity, | {constants:{Vc:0.006666667,Vs:(1-0.006666667),pro:0,ars2T:0,proK:16.605e-9},time:Infinity, | ||
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{constants:{Vc:0.000808081,Vs:(1-0.000808081),ars2T:0},time:Infinity, | {constants:{Vc:0.000808081,Vs:(1-0.000808081),ars2T:0},time:Infinity, | ||
data:{AsinT:[0.167289553e-6,0.185086314e-6,0.398647446e-6,0.473393842e-6], | data:{AsinT:[0.167289553e-6,0.185086314e-6,0.398647446e-6,0.473393842e-6], | ||
| - | AsT:[10e-6,20e-6,50e-6,100e-6]}}, | + | AsT:[10e-6,20e-6,50e-6,100e-6]}},*/ |
pSB1A2time: // concentration mode this is our first icps measerment wild type | pSB1A2time: // concentration mode this is our first icps measerment wild type | ||
{constants:{Vc:0.002320346,Vs:(1-0.002320346),ars2T:0},AsT:10e-6, | {constants:{Vc:0.002320346,Vs:(1-0.002320346),ars2T:0},AsT:10e-6, | ||
Revision as of 15:42, 12 October 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
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- Modelling
- DetailedModel
- Characterization
- Downloads
TODO: Talk about the devices we have and in what way we want to characterize them.
Uptake measurements
| Time (min) | ||||||
|---|---|---|---|---|---|---|
| 0 | 10 | 20 | 40 | 60 | ||
| As(III)exT(0) (µM) | 0 | x | ||||
| 10 | x | x | x | x | x | |
| 20 | x | |||||
| 50 | x | |||||
| 100 | x | |||||
To efficiently look at both time and concentration dependent processes we took samples as in the table on the right. Below we list all results, which have been used for fitting all necessary parameters.
TODO: List results. Take conversion from nmol/mg and mg/ml to µM and Vc/Vs into account.
| best | cur | gradient | solved | |
|---|---|---|---|---|
| v5/K5 | ||||
| v5 | ||||
| K5 | ||||
| k8/K7 | ||||
| k8 | ||||
| K7 | ||||
| tauBbeta4 | ||||
| tauB | ||||
| beta4 | ||||
| tauR | ||||
| beta1 | ||||
| E |
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Retrieved from "http://2009.igem.org/Team:Groningen/Modelling/Characterization"
