Team:Brown/Notebook Meetings/4-22-09

From 2009.igem.org

(Difference between revisions)
(New page: {{Brown}} iGEM 2009 Meeting April 22, 2009 9:40PM: Meeting begins in the SciLi Basement 9:43: iGEM account, Google groups account, Mathworks; email Neil for google group invitation!...)
 
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iGEM 2009 Meeting
+
'''iGEM 2009 Meeting'''
-
April 22, 2009
+
 
-
9:40PM: Meeting begins in the SciLi Basement  
+
'''April 22, 2009'''
-
9:43: iGEM account, Google groups account, Mathworks; email Neil for google group invitation!
+
 
-
9:45: Minoo and Eli are attending the Webinar
+
*9:40PM: Meeting begins in the SciLi Basement
-
9:46: Michael gives presentation on Bacterial Pixel  
+
*9:43: iGEM account, Google groups account, Mathworks; email Neil for google group invitation!
-
GFP, BFP, RGP components within a bacteria
+
*9:45: Minoo and Eli are attending the Webinar
-
Wavelength promoters need to be cloned
+
*9:46: Michael gives presentation on Bacterial Pixel  
-
10:00 Flora’s project: ability to detect cancer cells, then induce apoptosis
+
**GFP, BFP, RGP components within a bacteria
-
Detecting target – binding site on breast cancer cells – using nanoparticles to identify
+
**Wavelength promoters need to be cloned
-
Once cells are identified, induce apoptosis by bringing inducer molecule  
+
 
-
Flora will meet with them on Friday; report back; “Click Chemistry”
+
*10:00 Flora’s project: ability to detect cancer cells, then induce apoptosis
-
10:12: Indu & Minoo: prevention of allergies creating bi-functional protein – idenitify histamine & induce liver uptake
+
**Detecting target – binding site on breast cancer cells – using nanoparticles to identify
-
Common allergies - too much histamine
+
**Once cells are identified, induce apoptosis by bringing inducer molecule  
-
Tics secrete 3 binding histamine protein – have been cloned  
+
**Flora will meet with them on Friday; report back; “Click Chemistry”
-
EV131 in mice – binds to histamine with high affinity  
+
 
-
Look into mechanism to fuse these 2 proteins  
+
*10:12: Indu & Minoo: prevention of allergies creating bi-functional protein – idenitify histamine & induce liver uptake
-
10:25: Will – unlike every other team on the planet let’s use RNA synthetic biology
+
**Common allergies - too much histamine
-
RNA strand with UTR and translated region
+
**Tics secrete 3 binding histamine protein – have been cloned  
-
Toxin trigger conformational change of RNA  
+
**EV131 in mice – binds to histamine with high affinity  
-
Make RNA that binds to RNA strand, blocking ribosome binding
+
**Look into mechanism to fuse these 2 proteins  
-
Another part of RNA has spot that binds to one molecule, allowing RNA strand to fall off
+
 
-
Advantage: faster mechanism of gene regulation
+
*10:25: Will – unlike every other team on the planet let’s use RNA synthetic biology
-
“Ribo-regulators”
+
**RNA strand with UTR and translated region
-
10:38: Ahmad – specialized Safe Cell where dangerous cells will die if they escape
+
**Toxin trigger conformational change of RNA  
-
Cell can only produce ATP on plate
+
**Make RNA that binds to RNA strand, blocking ribosome binding
-
Uncouplers stop ATP synthesis  
+
**Another part of RNA has spot that binds to one molecule, allowing RNA strand to fall off
-
Inhibitor of uncoupler – liver x receptor alpha in media – cell dies  
+
**Advantage: faster mechanism of gene regulation
-
10:48: Ashley – eat as much as you want!
+
**“Ribo-regulators”
-
Leptin creates satiety signals, but obese individuals have large adipose, and large leptin; however, leptin receptors are not receptive  
+
 
-
Use leptin signal to break down fats directly  
+
*10:38: Ahmad – specialized Safe Cell where dangerous cells will die if they escape
-
Establish threshold leptin level  
+
**Cell can only produce ATP on plate
-
10:59: Stephanie – meningitis – bacteria has gene called lactate permease that when absent in mutant, mutant incapable of taking up lactose
+
**Uncouplers stop ATP synthesis  
-
Uses lactose instead of glucose as primary energy source
+
**Inhibitor of uncoupler – liver x receptor alpha in media – cell dies  
-
Bind something to lactose to prevent uptake
+
 
-
Needs adhesins
+
*10:48: Ashley – eat as much as you want!
-
Build machine that recognizes bacteria then have gene expressed where lactose binding lactin => lactate broken down
+
**Leptin creates satiety signals, but obese individuals have large adipose, and large leptin; however, leptin receptors are not receptive  
-
Proposed targeting this towards lactose intolerance  
+
**Use leptin signal to break down fats directly  
-
11:08: Eli – sphingosine and sphingosine 1-phosphate (signaling lipids)
+
**Establish threshold leptin level  
-
Spingosine is pro-apoptosis, anti-mitotic
+
 
-
Spingosine 1-P is anti-apoptosis, mitogenic (overexpressed in cancer cells)
+
*10:59: Stephanie – meningitis – bacteria has gene called lactate permease that when absent in mutant, mutant incapable of taking up lactose
-
Create switch that would induce cells to grow and die via ribo-regulator or regulation of transcription  
+
**Uses lactose instead of glucose as primary energy source
-
11:16: Need to pick favorites
+
**Bind something to lactose to prevent uptake
-
11:22: Everyone agrees on Bactopixel, Cancer-detection, and Histamine
+
**Needs adhesins
-
Possibility to implicate RNA regulators into each project  
+
**Build machine that recognizes bacteria then have gene expressed where lactose binding lactin => lactate broken down
-
Glue idea (ask Katrina)  
+
**Proposed targeting this towards lactose intolerance  
-
11:27: Next meeting 9:30 to 11:30 Same Place
+
 
-
TEAMS
+
*11:08: Eli – sphingosine and sphingosine 1-phosphate (signaling lipids)
-
Steph & Will – ribo-regulators
+
**Spingosine is pro-apoptosis, anti-mitotic
-
Will – Glue
+
**Spingosine 1-P is anti-apoptosis, mitogenic (overexpressed in cancer cells)
-
Minoo & Indu & Ashley – histamine  
+
**Create switch that would induce cells to grow and die via ribo-regulator or regulation of transcription  
-
Bactopixel – Michael & Eli
+
 
-
Cancer – Flora & Ahmad
+
*11:16: Need to pick favorites
 +
 
 +
*11:22: Everyone agrees on Bactopixel, Cancer-detection, and Histamine
 +
**Possibility to implicate RNA regulators into each project  
 +
**Glue idea (ask Katrina)  
 +
 
 +
*11:27: Next meeting 9:30 to 11:30 Same Place
 +
 
 +
*TEAMS
 +
**Steph & Will – ribo-regulators
 +
**Will – Glue
 +
**Minoo & Indu & Ashley – histamine  
 +
**Bactopixel – Michael & Eli
 +
**Cancer – Flora & Ahmad

Latest revision as of 02:09, 22 October 2009




iGEM 2009 Meeting

April 22, 2009

  • 9:40PM: Meeting begins in the SciLi Basement
  • 9:43: iGEM account, Google groups account, Mathworks; email Neil for google group invitation!
  • 9:45: Minoo and Eli are attending the Webinar
  • 9:46: Michael gives presentation on Bacterial Pixel
    • GFP, BFP, RGP components within a bacteria
    • Wavelength promoters need to be cloned
  • 10:00 Flora’s project: ability to detect cancer cells, then induce apoptosis
    • Detecting target – binding site on breast cancer cells – using nanoparticles to identify
    • Once cells are identified, induce apoptosis by bringing inducer molecule
    • Flora will meet with them on Friday; report back; “Click Chemistry”
  • 10:12: Indu & Minoo: prevention of allergies creating bi-functional protein – idenitify histamine & induce liver uptake
    • Common allergies - too much histamine
    • Tics secrete 3 binding histamine protein – have been cloned
    • EV131 in mice – binds to histamine with high affinity
    • Look into mechanism to fuse these 2 proteins
  • 10:25: Will – unlike every other team on the planet let’s use RNA synthetic biology
    • RNA strand with UTR and translated region
    • Toxin trigger conformational change of RNA
    • Make RNA that binds to RNA strand, blocking ribosome binding
    • Another part of RNA has spot that binds to one molecule, allowing RNA strand to fall off
    • Advantage: faster mechanism of gene regulation
    • “Ribo-regulators”
  • 10:38: Ahmad – specialized Safe Cell where dangerous cells will die if they escape
    • Cell can only produce ATP on plate
    • Uncouplers stop ATP synthesis
    • Inhibitor of uncoupler – liver x receptor alpha in media – cell dies
  • 10:48: Ashley – eat as much as you want!
    • Leptin creates satiety signals, but obese individuals have large adipose, and large leptin; however, leptin receptors are not receptive
    • Use leptin signal to break down fats directly
    • Establish threshold leptin level
  • 10:59: Stephanie – meningitis – bacteria has gene called lactate permease that when absent in mutant, mutant incapable of taking up lactose
    • Uses lactose instead of glucose as primary energy source
    • Bind something to lactose to prevent uptake
    • Needs adhesins
    • Build machine that recognizes bacteria then have gene expressed where lactose binding lactin => lactate broken down
    • Proposed targeting this towards lactose intolerance
  • 11:08: Eli – sphingosine and sphingosine 1-phosphate (signaling lipids)
    • Spingosine is pro-apoptosis, anti-mitotic
    • Spingosine 1-P is anti-apoptosis, mitogenic (overexpressed in cancer cells)
    • Create switch that would induce cells to grow and die via ribo-regulator or regulation of transcription
  • 11:16: Need to pick favorites
  • 11:22: Everyone agrees on Bactopixel, Cancer-detection, and Histamine
    • Possibility to implicate RNA regulators into each project
    • Glue idea (ask Katrina)
  • 11:27: Next meeting 9:30 to 11:30 Same Place
  • TEAMS
    • Steph & Will – ribo-regulators
    • Will – Glue
    • Minoo & Indu & Ashley – histamine
    • Bactopixel – Michael & Eli
    • Cancer – Flora & Ahmad
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