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Team:Newcastle/Labwork/9 October 2009
From 2009.igem.org
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* Prepared our biobricks to send for sequencing. Measured the concentration of the midipreps for our work. | * Prepared our biobricks to send for sequencing. Measured the concentration of the midipreps for our work. | ||
* Diluted the primers for pSB1AT3 | * Diluted the primers for pSB1AT3 | ||
| - | * Digest yesterday's Mini prep results | + | * Digest yesterday's Mini prep results |
| + | * Gel extraction for cotC PCR fragment after digestion, 4 sample's from Jen's promoter library experiment. | ||
== Conclusion == | == Conclusion == | ||
| - | * | + | * We checked the colonies on starch plate with iodine. We had successful transformation of pGFP-rrnB into ''B. subtilis'' however after the microscopy we realized that we did not have pMUTIN4 integrated into B. subtilis and we had to transform the colonies with pMUTIN4 for IPTG to work. So that LacI would be expressed in the cells. |
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| + | [[Image:Team_Newcastle_iGEM_2009_09_10_09_1_Lab_1.jpg|350px|center]] | ||
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| + | * We picked some colonies for kinA transformation growed on LB+Amp plate, after mini prep and digestion, the result of digestion told us that the colonies growed on LB+Amp plate are right colonies. Even they should not grow on LB+Amp plates. | ||
{{:Team:Newcastle/Project/Labwork/CalTemplate}} | {{:Team:Newcastle/Project/Labwork/CalTemplate}} | ||
Latest revision as of 18:27, 21 October 2009
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Formal Lab Session - 9th October 2009
Introduction
- Prepared our biobricks to send for sequencing. Measured the concentration of the midipreps for our work.
- Diluted the primers for pSB1AT3
- Digest yesterday's Mini prep results
- Gel extraction for cotC PCR fragment after digestion, 4 sample's from Jen's promoter library experiment.
Conclusion
- We checked the colonies on starch plate with iodine. We had successful transformation of pGFP-rrnB into B. subtilis however after the microscopy we realized that we did not have pMUTIN4 integrated into B. subtilis and we had to transform the colonies with pMUTIN4 for IPTG to work. So that LacI would be expressed in the cells.
- We picked some colonies for kinA transformation growed on LB+Amp plate, after mini prep and digestion, the result of digestion told us that the colonies growed on LB+Amp plate are right colonies. Even they should not grow on LB+Amp plates.
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News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
