Team:PKU Beijing/Parts Characterization

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==='''Characterization of the inducible promoters'''===
==='''Characterization of the inducible promoters'''===
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[[Image:PKU GRC AraC 120min.png|400px|left]][[Image:PKU_BBa_K228004_All6_new.png|400px|right]]
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[[Image:PKU GRC AraC 120min.png|355px|left]] [[Image:PKU_BBa_K228004_All6_new.png|355px|right]]
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By assembled with their activators/repressors upstream, inducible promoters may detect the existence of inducers such as arabinose and salicylate. The promoters here act as '''Small Molecule Sensor'''. Two favourite promoter parts involved have been very well characterized.
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(Learn more about the [[Team:PKU Beijing/Project/AND Gate 1|Small Molecule Sensor]])
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FOR MORE, PLEASE LOOK AT <br>[[Team:PKU Beijing/Parts Characterization/BBa K228004|'''Salicylate Promoter BBa_K228004''']] / [[Team:PKU Beijing/Parts Characterization/BBa K228009|'''Pbad Promoter BBa_K228009''']] Pages.
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We defined a new divice --- '''Small Molecule Sensor'''. By packaging inducible promoters and their activators/repressors, these device can detect the existence of inducers such as arabinose, IPTG.
 
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Some of the sensors are very well characterized. Figure on the right is the salicylate sensor, we use it as one example of the sensors.(Learn more about the [[Team:PKU_Beijing/Parts_Characterization/BBa_K228004|salicylate sensor]])
 
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FOR MORE, PLEASE LOOK AT <br>[[Team:PKU_Beijing/Project/AND_Gate_1/Design#Sensor_Module_.28Connected_to_the_input_interface_of_the_first_AND_Gate.29|'''Sensor Design''']] / [[Team:PKU_Beijing/Project/AND_Gate_1/Inducible_System_Result|'''Sensor Result''']] Pages.
 
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==='''Characterization of the AND Gate Parts'''===
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[[Image:PKU_AND_Gate_Mechanism.png|340px|left|thumb|Fig1. '''The mechanism of the AND Gate''' J Christopher Anderson, Christopher A Voigt and Adam P Arkin (2007) ''Environmental signal integration by a modular AND gate'' '''Molecular Systems Biology 3:133''']] [[Image: PKU_2K_AND_Gate.png|350px|right]]
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Our AND Gate is designed by J. Anderson, C. Voigt and A. Arkin (Molecular Systems Biology 3:133, 2007). We reconstructed the AND gate according to the principle of standard assembly, and finally greatly improved its efficiency. The AND gate senses small molecules as inputs and integrates the signals to activate T7 promoter, so as to trigger the expression of downstream gene. Different promoters are used to drive the expression of T7ptag and SupD tRNA to make this AND Gate. It really needs a great amount of work in order to find one qualified AND Gate out of tens of AND Gates. The figure on the top left demonstrates the mechanism of our AND Gate. The figure on the top right denotes one of the best AND Gates we achieved in the summer. These two AND Gate parts have been well characterized.
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(Learn more about the [[Team:PKU Beijing/Project/AND Gate 1/Design|AND Gate 1 design]] and [[Team:PKU Beijing/Project/AND Gate 2 Design|AND Gate 2 design]])
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FOR MORE, PLEASE LOOK AT<BR>
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[[Team:PKU Beijing/Parts Characterization/BBa K228258|'''AND Gate BBa_K228258''']] / [[Team:PKU Beijing/Parts Characterization/BBa K228260|'''AND Gate BBa_K228260''']] Pages
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{{PKU_Beijing/Foot}}
{{PKU_Beijing/Foot}}

Latest revision as of 17:59, 21 October 2009
 

Characterization of the inducible promoters

PKU GRC AraC 120min.png
PKU BBa K228004 All6 new.png


By assembled with their activators/repressors upstream, inducible promoters may detect the existence of inducers such as arabinose and salicylate. The promoters here act as Small Molecule Sensor. Two favourite promoter parts involved have been very well characterized.
(Learn more about the Small Molecule Sensor)

FOR MORE, PLEASE LOOK AT
Salicylate Promoter BBa_K228004 / Pbad Promoter BBa_K228009 Pages.

Characterization of the AND Gate Parts

Fig1. The mechanism of the AND Gate J Christopher Anderson, Christopher A Voigt and Adam P Arkin (2007) Environmental signal integration by a modular AND gate Molecular Systems Biology 3:133
PKU 2K AND Gate.png



Our AND Gate is designed by J. Anderson, C. Voigt and A. Arkin (Molecular Systems Biology 3:133, 2007). We reconstructed the AND gate according to the principle of standard assembly, and finally greatly improved its efficiency. The AND gate senses small molecules as inputs and integrates the signals to activate T7 promoter, so as to trigger the expression of downstream gene. Different promoters are used to drive the expression of T7ptag and SupD tRNA to make this AND Gate. It really needs a great amount of work in order to find one qualified AND Gate out of tens of AND Gates. The figure on the top left demonstrates the mechanism of our AND Gate. The figure on the top right denotes one of the best AND Gates we achieved in the summer. These two AND Gate parts have been well characterized.
(Learn more about the AND Gate 1 design and AND Gate 2 design)

FOR MORE, PLEASE LOOK AT
AND Gate BBa_K228258 / AND Gate BBa_K228260 Pages



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