Team:KU Seoul/Parts

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=== Part ===
=== Part ===
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::'''Mercury sensor - Mercury inducing promoter''' Part:{{Part|BBa_I728456}} + amd gene({{Part|BBa_K271000}})  
+
:'''Mercury sensor - Mercury inducing promoter''' Part:{{Part|BBa_I728456}} + amd gene({{Part|BBa_K271000}})  
[[Image:amd_part.png]]
[[Image:amd_part.png]]
:Cadmium is an intense toxic heavy metal with no known biological function. At low cadmium concentrations, cells are able to adapt and resume growth after a period of stationary period. Promoter yodA is only transiently activated during cadmium-induced growth inhibition.  
:Cadmium is an intense toxic heavy metal with no known biological function. At low cadmium concentrations, cells are able to adapt and resume growth after a period of stationary period. Promoter yodA is only transiently activated during cadmium-induced growth inhibition.  
:Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having amide bonds which is hydrolyzed by the enzyme, producing acetate and p-aminophenol. Because p-Aminophenol show brown color, aryl acylamidase gene can be considered as reporter gene.  
:Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having amide bonds which is hydrolyzed by the enzyme, producing acetate and p-aminophenol. Because p-Aminophenol show brown color, aryl acylamidase gene can be considered as reporter gene.  
 +
:'''Heavy-metal detector {{Part|BBa_K271001}}'''
:'''Heavy-metal detector {{Part|BBa_K271001}}'''

Latest revision as of 15:51, 21 October 2009






Part

Mercury sensor - Mercury inducing promoter Part:[http://partsregistry.org/Part:BBa_I728456 BBa_I728456] + amd gene([http://partsregistry.org/Part:BBa_K271000 BBa_K271000])

Amd part.png

Cadmium is an intense toxic heavy metal with no known biological function. At low cadmium concentrations, cells are able to adapt and resume growth after a period of stationary period. Promoter yodA is only transiently activated during cadmium-induced growth inhibition.
Aryl acylamidase (EC 3.5.1.13; AAA) is an enzyme that acts on the amide bond between aryl- and acyl groups. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is also reversible. Acetaminophen(Tyrenol) is an aryl acyl compound having amide bonds which is hydrolyzed by the enzyme, producing acetate and p-aminophenol. Because p-Aminophenol show brown color, aryl acylamidase gene can be considered as reporter gene.


Heavy-metal detector [http://partsregistry.org/Part:BBa_K271001 BBa_K271001]
The promoter/operator region of the zntA gene of Escherichia coli. It shows that Zn(II) is the primary inducer of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the promoters of mercury resistance(mer) operons, including a long spacer region containing an inverted repeat sequence.
Zinc part.png



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