Latest revision as of 03:13, 22 October 2009

Many thanks for taking part in the tour of our project. Feel free to browse through the sections of our project in more depth, using the sidetabs on the left or the images below.

Achievements

Here is a summary of the achievements of the Imperial College 2009 team. For more information about each achievement, please click on the relevant links below:

BioBricks

Submitted 20 documented parts to the Registry.
Improved the characterisation of the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200022 BBa_K098995 thermosensitive promoter] by successfully testing with GFP.
Improved the characterisation of the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200018 Part:BBa_K118011 glucose-sensitive promoter] by successfully testing with GFP.
Submitted new coding regions for 2 enzymes, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200008 PAH] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200007 cellulase], as well as a pentapeptide, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200013 opiorphin].
Designed new BioBricks using novel genes coding for encapsulation of the cell.
Functional BioBrick [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200022 BBa_K200022] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200018 BBa_K200018] characterised, and results uploaded onto registry page.

Engineering

Explored the Manufacturing Considerations that we thought important to manufacture The E.ncapsulator as a viable product.
Conceptualised a novel engineering technique of integrating our 3 modules.

Ethics

Explored the current Ethical Issues in Synthetic Biology, as well as those specific to our project.
Took part in the Valencia ethics survey, and became the first team to win the Valencia Ethics gold medal.
Investigated the safety implications of the project as a whole.

Others

Helped the iGEM newcomers UCL by sending them a plate of Registry DNA kit plate from 2007.

These links may be useful to browse further through our results and achievements:

Major Results

Submitted Parts

Human Practices

Pill Manufacture

Genealogy

@@ Line 1: / Line 1: @@
 {{Imperial/09/TemplateTop}}
 [[Image:II09_Logo_Hello.gif|right|The E.ncapsulator|200px]] <br><br><br>
 <b> Many thanks for taking part in the tour of our project. Feel free to browse through the sections of our project in more depth, using the sidetabs on the left or the images below.</b>
 <br><br><br><br><br>
-=Major Results=
+=[[Image:ii09_trophy.png | 50px]]Achievements=
+Here is a summary of the achievements of the Imperial College 2009 team. For more information about each achievement, please click on the relevant links below:
-This page contains highlights of our major results and simulations. It is by no means complete. However, more results will be presented at the jamboree.
+===[[Image:ii09_brick.png | 50px]]BioBricks===
+* Submitted <b>[[Team:Imperial_College_London/Wetlab/BioBricks |20 documented parts]]</b> to the Registry.
+* <b>Improved the characterisation</b> of the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200022 BBa_K098995 thermosensitive promoter] by successfully testing with GFP.
+*<b>Improved the characterisation</b> of the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200018 Part:BBa_K118011 glucose-sensitive promoter] by successfully testing with GFP.
+* Submitted <b>new coding regions</b> for 2 enzymes, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200008 PAH] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200007 cellulase], as well as a pentapeptide, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200013 opiorphin].
+* <b>Designed new BioBricks</b> using [[Team:Imperial_College_London/M2 |novel genes]] coding for encapsulation of the cell.
+* <b>Functional BioBrick [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200022 BBa_K200022] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200018 BBa_K200018]  characterised</b>, and results uploaded onto registry page.
-==Simulations: Chemoinduction for <b>Module 1</b>==
+===[[Image:ii09_eng.PNG | 50px]]Engineering===
-Our [[Team:Imperial_College_London/Drylab/Protein_Production | simulation results]] indicate that <b>increasing input amounts of IPTG</b> result in a <b>greater yield</b> of polypeptide drug of interest.
+* Explored the [[Team:Imperial_College_London/Manufacturing_Considerations |Manufacturing Considerations]] that we thought important to manufacture <b><i>The E.ncapsulator</i></b> as a viable product.
+*Conceptualised a novel engineering technique of [[Team:Imperial_College_London/Temporal_Control | integrating]] our 3 modules.
-An experiment was done to investigate how different input concentrations of IPTG affect cell growth. [[https://2009.igem.org/Team:Imperial_College_London/Wetlab/Results/Cheminduction  | Our results]] show that IPTG has no significant effect on cell growth within the ranges we are using. Therefore, we can conclude that <b>increasing IPTG concentration initially will have a positive contribution to our dosage.</b>  <br>
+<br>
-[[Image:II09_SIm_main_prot.jpg]]<br>
-<b>Note 1:  Results from these simulations were to be tied up with data from our LacI-RFP testing construct (See our part  [https://2009.igem.org/Team:Imperial_College_London/Wetlab/BioBricks K200019]. Unluckily, this part of the construct was not sent correcty from the registry, so testing again was not possible before the wiki-freeze deadline.</b><br>
-<b>Note 2: Parameters used in the system are arbitrary. For a justification see the system's  [https://static.igem.org/mediawiki/2009/a/a0/II09_Prot_stability_analysis.pdf stability analysis.] </b>
-==Experimental: Module 2 - Encapsulation growth curves==
+===[[Image:ii09_eth.PNG | 50px]]Ethics===
-Dependent on lab results today
-==Experimental: Autoinduction - Diauxic growth curve and CRP GFP==
+* Explored the current [[Team:Imperial_College_London/Ethics |Ethical Issues]] in Synthetic Biology, as well as those specific to our project.
-Here we can pick a diauxic growth curve and compare directly to a simulation
+* Took part in the Valencia ethics survey, and became the first team to win the [[Team:Valencia/Human_Practice/Medalls |Valencia Ethics]] gold medal.
+* Investigated the [[Team:Imperial_College_London/SafetyConsiderations |safety implications]] of the project as a whole.
-==Simulations: Module 1 - Enzyme kinetics and dosage control==
+<br>
-[[Image:II09_enzymekinetics.png|330px|left]]<br>
-Many polypeptides of interest are enzymes. This means that detecting how much drug has been produced requires knowledge about their interaction with their respective substrates in the enzymatic assays. Here we assumed that enzymatic activity follows Michaelis-Menten kinetics [1-3]. This means that we can directly relate enzymatic activity to the required dosage for successful administration of the polypeptide of interest. <br>
-<b>Dosage Calculations:</b><br>
-//upload summary and complete pdf file.<br><br>
-==Experimental: Module 3 - Thermoinducible promoter==
+===[[Image:ii09_tick.PNG | 40px]]Others===
-Need Matthieu's latest processed data
-=Conclusion=
-Need some inspiration here guys!
-= Achievements=
-Here is a summary of the achievements of the Imperial College 2009 team:
-[[Image:ii09_trophy.png | right | 300px]]
-* Submitted <b>[[Team:Imperial_College_London/Wetlab/BioBricks |20 documented parts]]</b> to the Registry.
-* <b>Improved the characterisation</b> of the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200022 BBa_K098995 thermosensitive promoter] by successfully testing with GFP.
-* Submitted <b>new coding regions</b> for 2 enzymes, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200008 PAH] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200007 cellulase], as well as a pentapeptide, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200013 opiorphin].
-* <b>Designed new BioBricks</b> using those [[Team:Imperial_College_London/M2 |genes]] coding for encapsulation of the cell.
-* <b>Functional BioBrick [http://partsregistry.org/wiki/index.php?title=Part:BBa_K200022 BBa_K200022] characterised</b>, and results uploaded onto registry page.
-* Explored the current [[Team:Imperial_College_London/Ethics |Ethical Issues]] in Synthetic Biology, as well as those specific to our project.
 * <b>Helped the iGEM newcomers</b> [[Team:UCL_London |UCL]] by sending them a plate of Registry DNA kit plate from 2007.
-* Took part in the Valencia ethics survey, and became the first team to win the [[Team:Valencia/Human_Practice/Medalls |Valencia Ethics]] gold medal.
-* Explored the [[Team:Imperial_College_London/Manufacturing_Considerations |Manufacturing Considerations]] that we thought important to manufacture <b><i>The E.ncapsulator</i></b> as a viable product.
-* Investigated the [[Team:Imperial_College_London/SafetyConsiderations |Safety Implications]] of the project as a whole.
 <br><br><br><br><br>
+<hr>
 <center><b>These links may be useful to browse further through our results and achievements: </b></center>
 <html><center><a href="https://2009.igem.org/Team:Imperial_College_London/Major_results"><img style="vertical-align:bottom;" width="19%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage2.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Wetlab/BioBricks"><img style="vertical-align:bottom;" width="19%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage3.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Ethics"><img style="vertical-align:bottom;" width="19%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage4.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Manufacturing_Considerations"><img style="vertical-align:bottom;" width="19%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Homepageimage5.png"></a><html><a
 href="https://2009.igem.org/Team:Imperial_College_London/genealogy"><img style="vertical-align:bottom;" width="23%" src="https://static.igem.org/mediawiki/2009/2/20/II09_WorldMap.png"></a><center></html>
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Team:Imperial College London/Summary

From 2009.igem.org