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Team:PKU Beijing/Parts Characterization
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==='''Characterization of the inducible promoters'''=== | ==='''Characterization of the inducible promoters'''=== | ||
| - | [[Image:PKU GRC AraC 120min.png| | + | [[Image:PKU GRC AraC 120min.png|355px|left]] [[Image:PKU_BBa_K228004_All6_new.png|355px|right]] |
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By assembled with their activators/repressors upstream, inducible promoters may detect the existence of inducers such as arabinose and salicylate. The promoters here act as '''Small Molecule Sensor'''. Two favourite promoter parts involved have been very well characterized. | By assembled with their activators/repressors upstream, inducible promoters may detect the existence of inducers such as arabinose and salicylate. The promoters here act as '''Small Molecule Sensor'''. Two favourite promoter parts involved have been very well characterized. | ||
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(Learn more about the [[Team:PKU Beijing/Project/AND Gate 1|Small Molecule Sensor]]) | (Learn more about the [[Team:PKU Beijing/Project/AND Gate 1|Small Molecule Sensor]]) | ||
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| - | FOR MORE, PLEASE LOOK AT <br>[[Team:PKU Beijing/Parts Characterization/BBa K228004|'''Salicylate Promoter''']] / [[Team:PKU Beijing/Parts Characterization/BBa K228009|'''Pbad Promoter''']] Pages. | + | FOR MORE, PLEASE LOOK AT <br>[[Team:PKU Beijing/Parts Characterization/BBa K228004|'''Salicylate Promoter BBa_K228004''']] / [[Team:PKU Beijing/Parts Characterization/BBa K228009|'''Pbad Promoter BBa_K228009''']] Pages. |
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==='''Characterization of the AND Gate Parts'''=== | ==='''Characterization of the AND Gate Parts'''=== | ||
| - | + | [[Image:PKU_AND_Gate_Mechanism.png|340px|left|thumb|Fig1. '''The mechanism of the AND Gate''' J Christopher Anderson, Christopher A Voigt and Adam P Arkin (2007) ''Environmental signal integration by a modular AND gate'' '''Molecular Systems Biology 3:133''']] [[Image: PKU_2K_AND_Gate.png|350px|right]] | |
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| + | Our AND Gate is designed by J. Anderson, C. Voigt and A. Arkin (Molecular Systems Biology 3:133, 2007). We reconstructed the AND gate according to the principle of standard assembly, and finally greatly improved its efficiency. The AND gate senses small molecules as inputs and integrates the signals to activate T7 promoter, so as to trigger the expression of downstream gene. Different promoters are used to drive the expression of T7ptag and SupD tRNA to make this AND Gate. It really needs a great amount of work in order to find one qualified AND Gate out of tens of AND Gates. The figure on the top left demonstrates the mechanism of our AND Gate. The figure on the top right denotes one of the best AND Gates we achieved in the summer. These two AND Gate parts have been well characterized. | ||
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| + | (Learn more about the [[Team:PKU Beijing/Project/AND Gate 1/Design|AND Gate 1 design]] and [[Team:PKU Beijing/Project/AND Gate 2 Design|AND Gate 2 design]]) | ||
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| + | FOR MORE, PLEASE LOOK AT<BR> | ||
| + | [[Team:PKU Beijing/Parts Characterization/BBa K228258|'''AND Gate BBa_K228258''']] / [[Team:PKU Beijing/Parts Characterization/BBa K228260|'''AND Gate BBa_K228260''']] Pages | ||
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{{PKU_Beijing/Foot}} | {{PKU_Beijing/Foot}} | ||
Latest revision as of 17:59, 21 October 2009
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