This page contains our experimental results. If there are any further results we obtained from after the wiki freeze, they will be put on our [http://openwetware.org/wiki/IGEM:NYMU NYMU openwetware website].
| Part Name | Picture | Part Type | Part Length | Subparts | Short Description (max 60 chars) | Long Description (what it is, what it does, how to use) | Design Notes | Source (where does it come from?)
|
| BBa_K195300 |
neu gene operon
K195300
 |
| coding | 5229 | BBa_K195300 | This part is composed of neuD,neuB,neuA,neuC,neuE(neu gene cluster). | The coding sequence neu genes are operate in one operon in E.coli K1, which are coding for the enzymes that are involved in sialic acid synthesis.
| | NCKU provided us the E.coli K1.
|
| BBa_K195301 |
K195301
neu gene operon
K195300
|
Terminator
B0015
 |
| composite | | BBa_K195300 BBa_B0015 | This part is composed of neu gene operon and terminator. |
| |
|
| BBa_K195302 |
| generator | | BBa_B0034 BBa_K195300 BBa_B0015 | This part is composed of RBS,neu gene operon and terminator. | The coding sequence aim at sialic acid synthesis.
| |
|
| BBa_K195303 |
| generator | | BBa_R0040 BBa_B0034 BBa_K195300 BBa_B0015 | This part is composed of pTet, RBS, neu gene operon and terminator. | The pTet promoter is strong enough to overexpress the neu gene operon.
| |
|
| BBa_K195304 |
sialyltransferase 0160
K195304
|
| coding | 2006 | BBa_K195304 | This part is the coding sequence of alpha-2,6-sialyltransferase. | alpha-2,6-sialyltransferase can transfer polysaccharides onto the cell membrane to fuse with the membrane proteins, which then form glycoproteins.
|
| BBa_K195305 |
K195305
sialyltransferase 0160
K195304
|
Terminator
B0015
|
| composite | | BBa_K195304 BBa_B0015 | This part is composed of sialyltransferase 0160 and terminator. |
| |
|
| BBa_K195306 |
| generator | | BBa_B0034 BBa_K195304 BBa_B0015 | This part is composed of RBS,sialyltransferase 0160 and terminator. |
| |
|
| BBa_K195307 |
| generator | | BBa_R0040 BBa_B0034 BBa_K195304 BBa_B0015 | This part is composed of pTet, RBS, sialyltransferase 0160 and terminator. | The pTet promoter is strong enough to overexpress the sialyltransferase 0160.
| |
|
| Part Name | Picture | Part Type | Part Length | Subparts | Short Description (max 60 chars) | Long Description (what it is, what it does, how to use) | Design Notes | Source (where does it come from?)
|
| BBa_K195400 |
F10scFv
K195400
|
| Coding | 840 | K195400 | single chain Fv (Antibody) that bind to Influenza | F10scFv can bind to the highly conserved region in HA on many subtypes of the Influenza A virus. F10scFv binds to a common epitope that is a highly conserved pocket in the stem region of Hemagglutinin containing the fusion peptide.
|
| synthesis by Mr.gene
|
| BBa_K195401 |
K195401
Lpp-OmpA-linker
K103006
|
F10scFv
K195400
|
| composite | 1304 | BBa_K103006 BBa_K195400 | Fusion BBa_K195400 with BBa_K103006 | Fusion our BBa_K195400 with BBa_K103006, so the F10scFv can be anchored on surface of E. coli.
| Scarless in-fusion by PCR
|
|
| BBa_K195402 |
| generator | 1332 | BBa_B0034 BBa_K103006 BBa_K195400 | This part is composed of RBS,Lpp-OmpA-linker and F10scFv | The coding sequence can express F10scFv(BBa_K195400) on outer membrane by OmpA(BBa_K103006).
|
|
|
| BBa_K195403 |
| generator | 1384 | BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195400 | This part is composed of pTetR,RBS,Lpp-OmpA-linker and F10scFv | Fusion our BBa_K195400 with BBa_K103006, so our F10scFv can be transported to outer membrane of E. coli.
|
|
|
| BBa_K195404 |
| generator | 1531 | BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195400 BBa_B0015 | This part is composed of pTetR,RBS,Lpp-OmpA-linker,F10scFv and terminator | Fusion our BBa_K195400 with BBa_K103006, so our F10scFv can be transported to outer membrane of E. coli.
|
|
|
| BBa_K195410 |
C-IgAP
K195405
|
| coding | 1257 | BBa_K195405 | C-terminal of IgA protease | The leader sequence of C-IgAP is cleaved at the membrane by a signal peptidase, releasing the mature polyprotein into the periplasm. When in the periplasm, the beta-domain of the protein inserts into the outer membrane to form a beta-barrel structure. After formation of the beta-barrel pore, the passenger domain is translocated to the cell surface through the pore. Once at the cell surface, several possibilities may occur.
|
| Neisseria gonorrhoeae FA1090(provide by Dr.Hsing-Ju Wu, Academia sinica/Institute of Biological Chemistry)
|
| BBa_K195406 |
K195406
F10scFv
K195400
|
C-IgAP
K195406
|
| composite | 2097 | BBa_K195400 BBa_K195406 | This part is composed of F10scFv and C-IgAP | scFv fusion with C-IgAP
|
|
|
| BBa_K195407 |
K195407
PelB
J32015
|
F10scFv
K195400
|
| composite | 906 | BBa_J32015 BBa_K195400 | This part is composed of PelB and F10scFv | scFv fusion with PelB
|
|
|
| BBa_K195408 |
| composite | 2163 | BBa_J32015 BBa_K195400 BBa_K195406 | This part is composed of PelB,F10scFv and C-IgAP |
|
|
| BBa_K195409 |
| generator | 2197 | BBa_B0034 BBa_J32015 BBa_K195400 BBa_K195406 | This part is composed of RBS,PelB,F10scFv and C-IgAP | The coding sequence express F10scFv on E.coli surface
|
|
|
| BBa_K195412 |
| generator | 2334 | BBa_B0034 BBa_J32015 BBa_K195400 BBa_K195406 BBa_B0015 | This part is composed of RBS,PelB,F10scFv,C-IgAP and terminator | The coding sequence express F10scFv on E.coli surface
|
|
| BBa_K195411 |
| generator | 2396 | BBa_B0034 BBa_J32015 BBa_K195400 BBa_K195406 BBa_B0015 | This part is composed of pTetR,RBS,PelB,F10scFv,C-IgAP and terminator | The coding sequence express F10scFv on E.coli surface
|
|
| Part Name | Picture | Part Type | Part Length | Subparts | Short Description (max 60 chars) | Long Description (what it is, what it does, how to use) | Design Notes | Source (where does it come from?)
|
| BBa_K195600 |
K195600
CII
K116602
|
Terminator
B0015
|
| composite | 431 | BBa_K116602 BBa_B0015 | This part is composed of CII and terminator. | The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage.
| | This part comes from IGEM_08 NYMU-Taipei.
|
| BBa_K195601 |
| generator | 449 | BBa_B0034 BBa_K116602 BBa_B0015 | This part is composed of RBS,CII and terminator. | The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage.
| |
|
| BBa_K195602 |
K195602
TetR
C0040
|
Terminator
B0015
|
| composite | 822 | BBa_C0040 BBa_B0015 | This part is composed of TetR and terminator. | The coding sequence TetR could repress the regulatory promoter pTetR(BBa_R0040).
| |
|
| BBa_K195603 |
| generator | 840 | BBa_B0034 BBa_C0040 BBa_B0015 | This part is composed of RBS,TetR and terminator. | The coding sequence TetR could repress the regulatory promoter pTet(BBa_R0040).
| |
|
| BBa_K195604 |
K195604
PenI
C0074
|
Terminator
B0015
|
| composite | 585 | BBa_C0074 BBa_B0015 | This part is composed of PenI and terminator. | The coding sequence PenI could repress regulatory promoter pPenI(BBa_R0074).
| |
|
| BBa_K195605 |
| generator | 603 | BBa_B0034 BBa_C0074 BBa_B0015 | This part is composed of RBS,PenI and terminator. | The coding sequence PenI could repress regulatory promoter pPenI(BBa_R0074).
| |
|
| BBa_K195606 |
| generator | 511 | BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015 | This part is composed of pTetR, RBS,CII and terminator. | The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The TetR repressible promoter is repressed by TetR (BBa_C0040).
| |
|
| BBa_K195607 |
| generator | 534 | BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 | This part is composed of pPenI, RBS,CII and terminator. | The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The PenI repressible promoter is repressed by PenI (BBa_C0074).
| |
|
| BBa_K195608 |
| generator | 657 | BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015 | This part is composed of pLacI, RBS,CII and terminator. | The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage.
| |
|
| BBa_K195609 |
| Reporter | 931 | BBa_K116603 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 | This part is composed of pRE and GFP generator. | The promoter pRE could be activated by BBa_K116602,the activator CII.
| |
|
| BBa_K195610 |
| Reporter | 937 | BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 | This part is composed of pTet and GFP generator. | The promoter pTet could be repressed by BBa_C0040,the repressor TetR.
| |
|
| BBa_K195611 |
| Reporter | 960 | BBa_R0074 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 | This part is composed of pPenI and GFP generator. | The promoter pPenI could be repressed by BBa_C0074,the repressor PenI.
| |
|
| BBa_K195612 |
| Reporter | 1083 | BBa_R0010 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 | This part is composed of pLacI and GFP generator. | The pLacI is repressed by LacI, which could be inhibited by IPTG.
| |
|
| BBa_K195613 |
| Reporter | 932 | BBa_R1051 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 | This part is composed of pCI and GFP generator. | The promoter pCI could be repressed by BBa_C0051,the repressor CI from E.coli phage lambda.
| |
|
| BBa_K195614 |
| Reporter | 937 | BBa_R0053 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 | This part is composed of p22 and GFP generator. | The promoter p22 could be repressed by BBa_C0053,the c2 repressor from Salmonella phage P22 .
| |
|
| BBa_K195615 |
| Reporter | 938 | BBa_R0074 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 | This part is composed of pLuxR and GFP generator. | The promoter pLuxR could be activated by LuxR in concert with HSL.
| |
|
| BBa_K195616 |
| Reporter | 1040 | BBa_R0074 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015 | This part is composed of pLasR and GFP generator. | The promoter pLasR could be repressed by BBa_C0079,the repressor LasR.
| |
|
| BBa_K195617 |
| composite | 1450 | BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015 | This part is composed of pTetR with activator gene CII, and it would activate pRE to produce green fluorescent protein. | The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The TetR repressible promoter is repressed by TetR (BBa_C0040).The pRE would be activated by CII produced by the downstream gene of the regulatory promoter pTet and produce green fluorescent protein.
| |
|
| BBa_K195618 |
| composite | 1473 | BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015 | This part is composed of pPenI with activator gene CII, and it would activate pRE to produce green fluorescent protein. | The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The PenI repressible promoter is repressed by PenI (BBa_C0074).The pRE would be activated by CII produced by the downstream gene of the regulatory promoter pPenI and produce green fluorescent protein.
| |
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| BBa_K195619 |
| composite | 1596 | BBa_R0010 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015 | This part is composed of pLacI with activator gene CII, and it would activate pRE to produce green fluorescent protein. | The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The pLacI is repressed by LacI, which could be inhibited by IPTG.The pRE would be activated by CII produced by the downstream gene of the regulatory promoter pLacI and produce green fluorescent protein.
| |
|
| BBa_K195620 |
| generator | 896 | BBa_K116603 BBa_B0034 BBa_C0040 BBa_B0015 | This part is composed of pRE and TetR repressor. | The promoter pRE could be activated by BBa_K116602,the activator CII. TetR could repress the regulatory promoter BBa_R0040 pTet.
| |
|
| BBa_K195621 |
| generator | 659 | BBa_K116603 BBa_B0034 BBa_C0074 BBa_B0015 | This part is composed of pRE and PenI repressor. | The promoter pRE could be activated by BBa_K116602,the activator CII. PenI from Bacillus licheniformis with LVA tail could repress the regulatory promoter BBa_R0074 pPenI.
| |
|
| BBa_K195622 |
| Device | 2354 | BBa_K116603 BBa_B0034 BBa_C0040 BBa_B0015 BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015 | This part is a damped oscillator by using the regulatory promoter and the interaction between the activator, repressor and regulatory promoter. | This device is produces damped oscillations between TetR and CII. The pRE (K116603) can be activated by CII (K116602), which in turn produces TetR (C0040) and GFP. The TetR then represses the pTet promoter (R0040) resulting in slower production of CII.
This design was based on the design of a relaxation oscillator (two component oscillator). This design does not produce sustained oscillations like a normal oscillator (relaxation based or otherwise) because of the two oscillating effectors not being able to directly affect each other.
| |
|
| BBa_K195623 |
| Device | 2140 | BBa_K116603 BBa_B0034 BBa_C0074 BBa_B0015 BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015 | This part is a time delay device using the regulatory promoter and the interaction between the activator, repressor and regulatory promoter. | This device is produces damped oscillations between PenI and CII. The pRE (K116603) can be activated by CII (K116602), which in turn produces PenI (C0074) and GFP. The PenI then represses the pPenI promoter (R0074) resulting in slower production of CII. This design was based on the design of a relaxation oscillator (two component oscillator). This design does not produce sustained oscillations like a normal oscillator (relaxation based or otherwise) because of the two oscillating effectors not being able to directly affect each other.
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| BBa_K195624 |
| Device | 1415 | BBa_K116603 BBa_B0034 BBa_C0040 BBa_B0015 BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015 | This main part of the time delay device. | The promoter pRE could be activated by BBa_K116602,the activator CII, which results in the production of the TetR and green fluorescent protein. TetR could repress the regulatory promoter BBa_R0040 pTet, which results in the lower production of the activator CII.
| |
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| BBa_K195625 |
| Device | 1210 | BBa_K116603 BBa_B0034 BBa_C0074 BBa_B0015 BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 | This main part of the time delay device. | The promoter pRE could be activated by BBa_K116602,the activator CII, which results in the production of the TetR and green fluorescent protein. PenI from Bacillus licheniformis with LVA tail could repress the regulatory promoter BBa_R0074 pPenI, which results in the lower production of the activator CII.
| |
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| BBa_K195626 |
| generator | 665 | BBa_R0040 BBa_B0034 BBa_C0074 BBa_B0015 | This part is composed of pTetR, RBS,PenI and terminator. | The coding sequence PenI could repress BBa_R0074,the regulatory promoter pPenI. This is a subunit of toggle switch. pTet could be repressed by repressor TetR (BBa_C0040).
| |
|
| BBa_K195627 |
| generator | 925 | BBa_R0074 BBa_B0034 BBa_C0040 BBa_B0015 | This part is composed of pPenI, RBS,TetR and terminator. | The coding sequence TetR could repress BBa_R0040,the regulatory promoter pTet. This is a subunit of toggle switch. pPenI could be repressed by repressor PenI (BBa_C0074).
| |
|
| BBa_K195628 |
| Device | 1207 | BBa_R0040 BBa_B0034 BBa_C0074 BBa_B0015 BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 | This part is a toggle switch. | The coding sequence PenI could repress BBa_R0074,the regulatory promoter pPenI.The coding sequence TetR could repress BBa_R0040,the regulatory promoter pTet.
| |
|
| BBa_K195629 |
K195629
Cl lam
C0051
|
Terminator
B0015
|
| composite | 912 | BBa_C0051 BBa_B0015 | This part is composed of cl repressor and terminator. | The coding sequence cl repressor from bacteriophage lambda with LVA tail could repress BBa_R1051,the regulatory promoter pCI.
| |
|
| BBa_K195630 |
| generator | 930 | BBa_B0034 BBa_C0051 BBa_B0015 | This part is composed of RBS,cl repressor and terminator. | The coding sequence cl repressor from bacteriophage lambda with LVA tail could repress BBa_R1051,the regulatory promoter pCI.
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