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Team:Brown/Notebook Protocols/primerresusp
From 2009.igem.org
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'''<big>Primer resuspension</big>''' | '''<big>Primer resuspension</big>''' | ||
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Goal: 5pM/ul. This is a 1x stock solution. | Goal: 5pM/ul. This is a 1x stock solution. | ||
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Protocol: | Protocol: | ||
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1) Identify the nM amount on the tube and resuspend the primers in 10x (in ul) water, e.g. 23.5 nM would be resuspended in 235 uL water. This is a 100 pM (or 0.1 nM) and is the 20x that you protect! | 1) Identify the nM amount on the tube and resuspend the primers in 10x (in ul) water, e.g. 23.5 nM would be resuspended in 235 uL water. This is a 100 pM (or 0.1 nM) and is the 20x that you protect! | ||
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2) Dilute a portion of this 20x into a fresh tube, appropriately labeled, to make a 1X stock solution, or 5pm/uL. This is your working solution. Use 2uL per reaction. | 2) Dilute a portion of this 20x into a fresh tube, appropriately labeled, to make a 1X stock solution, or 5pm/uL. This is your working solution. Use 2uL per reaction. | ||
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3) Store at -20oC. | 3) Store at -20oC. | ||
Latest revision as of 23:01, 21 October 2009
Primer resuspension
Goal: 5pM/ul. This is a 1x stock solution.
Protocol:
1) Identify the nM amount on the tube and resuspend the primers in 10x (in ul) water, e.g. 23.5 nM would be resuspended in 235 uL water. This is a 100 pM (or 0.1 nM) and is the 20x that you protect!
2) Dilute a portion of this 20x into a fresh tube, appropriately labeled, to make a 1X stock solution, or 5pm/uL. This is your working solution. Use 2uL per reaction.
3) Store at -20oC.
