Team:Warsaw/Calendar-Main/10 July 2009

From 2009.igem.org

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__NOTOC__
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==Team meeting==
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<h3>Team meeting</h3>
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__NOEDITSECTION__
# presentations of work did by both groups during last week (given by Ania and Kuba)
# presentations of work did by both groups during last week (given by Ania and Kuba)
# presentation of methods used by teams starting in previous editions of iGEM to kill bacteria :) (Kamil)
# presentation of methods used by teams starting in previous editions of iGEM to kill bacteria :) (Kamil)
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<!-- TU EDYTUJE FRANEK, NIE RUSZ! -->
<!-- TU EDYTUJE FRANEK, NIE RUSZ! -->
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<html>
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===<div style="text-align: center;">Creating devices to test promoters in <em>E. coli</em> strains (devices [http://partsregistry.org/Part:BBa_K177024 <span style="color: black;">BBa_K177024</span>] and [http://partsregistry.org/Part:BBa_K177025 <span style="color: black;">BBa_K177025</span>])</div>===
-
<center><h3>Miecznikowa team: Creating devices to test promotors in <i>E. coli</i> strains (devices <a href=http://partsregistry.org/Part:BBa_K177024>BBa_K177024</a> and <a href=http://partsregistry.org/Part:BBa_K177025>BBa_K177025</a>)</h3></center>
+
 
-
<h4>Franek</h4>
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'''Franek'''
 +
 
<br>
<br>
-
<p>Task:</p>
+
Tasks:
-
<ul>
+
 
-
<li>Alkaline lysis of the plasmid containing <a href=http://partsregistry.org/Part:BBa_B0024>BBa_B0024</a></li>
+
* Alkaline lysis of the plasmid containing [http://partsregistry.org/Part:BBa_B0024 <span style="color: black;">BBa_B0024 terminator</span>]
-
<li>Setting cultures with <a href=http://partsregistry.org/Part:BBa_R0010>BBa_R0010</a> and <a href=http://partsregistry.org/Part:BBa_R0080>BBa_R0080</a></li>
+
-
</ul>
+
<br>
<br>
-
<p>Methods:</p>
+
Methods:
-
<ul>
+
 
-
<li>PlasmidMini set by <a href="http://aabiot.com/products/dna_purification/dna_fragments/gel_out/protocol_gel_out.pdf">A&A Biotechnology</a> was used. 2 test tubes with 5ml LB and ampicillin were first inoculated with colonies containing <a href=http://partsregistry.org/Part:BBa_B0024>BBa_B0024</a> brick on pSB1A2 plasmid. The cultures were incubated for 6h at 37&deg;C. Alkaline lysis was performed on both cultures. The pellet from 5 ml of bacteria was used. </li>
+
* [http://aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf <span style="color: black;">PlasmidMini set by A&A Biotechnology</span>] was used. 2 test tubes with 5ml LB and ampicillin were first inoculated with colonies containing [http://partsregistry.org/Part:BBa_B0024 <span style="color: black;">BBa_B0024</span>] brick on [http://partsregistry.org/Part:pSB1A2 <span style="color: black;">pSB1A2</span>] plasmid. The cultures were incubated for 6h at 37&deg;C. Alkaline lysis was performed on both cultures. The pellet from 5 ml of bacteria was used.  
-
<li>3 test tubes with 5ml LB and ampicillin were inoculated with colonies containing <a href=http://partsregistry.org/Part:BBa_R0010>BBa_R0010</a> brick on pSB1A2 plasmid. The cultures were left for over night incubation at 37&deg;C.</li>
+
<br>
-
<li>3 test tubes with 5ml LB and ampicillin were inoculated with colonies containing <a href=http://partsregistry.org/Part:BBa_R0080>BBa_R0080</a> brick on pSB1A2 plasmid. The cultures were left for overnight incubation at 37&deg;C.</li>
+
Results:
-
</ul>
+
 
 +
* Concentration of DNA sample was measured using NanoDrop ND-1000
<br>
<br>
-
<p>Results:</p>
 
-
<ul>
 
-
<li>Concentration of DNA sample was measured using NanoDrop ND-1000.</li>
 
-
</ul>
 
-
</html>
 
{| class="wikitable" style="text-align:center; width:400px; height:90px; border-collapse: collapse; border: 1px dashed blue; margin: 1em auto 1em auto" border="1"
{| class="wikitable" style="text-align:center; width:400px; height:90px; border-collapse: collapse; border: 1px dashed blue; margin: 1em auto 1em auto" border="1"
|-
|-
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!  DNA concentration in ng/&micro;l
!  DNA concentration in ng/&micro;l
|-
|-
-
|  BBa_B0024
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|  [http://partsregistry.org/Part:BBa_B0024 <span style="color: black;">BBa_B0024</span>]
|  33.82
|  33.82
|}
|}
-
<html>
 
<br>
<br>
-
<p>Notes:</p>
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<br>
-
<ul>
+
Notes:
-
<li>Plates with <a href=http://partsregistry.org/partsdb/get_part.cgi?part=BBa_I0500>BBa_I0500</a> transformants were empty once again. This result, together with note in <a href=http://partsregistry.org/partsdb/get_part.cgi?part=BBa_I0500>part description</a> suggest that this part is damaged.</li>
+
 
-
</ul>
+
* Plates with [http://partsregistry.org/Part:BBa_I0500 <span style="color: black;">BBa_I0500</span>] transformants were empty once again. This result, together with note in [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_I0500 <span style="color: black;">part description</span>] suggest that this part is damaged
-
</html>
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<!-- TU PISZ CO CHCESZ! -->
<!-- TU PISZ CO CHCESZ! -->
 +
===<div style="text-align: center;">Making of RBS-cI part</div>===
 +
<html>
<html>
<h4>Jarek</h4>
<h4>Jarek</h4>
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<p>Task:</p>
<p>Task:</p>
<ul>
<ul>
-
<li>Digestion of parts C0051 and B0032</li>
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<li>Digestion of parts <a href="http://partsregistry.org/Part:BBa_C0051"><span style="color: black">BBa_C0051</a></span> and <a href="http://partsregistry.org/Part:BBa_B0032"><span style="color: black">BBa_B0032</a></span></li>
<li>Electrophoretic separation of digested parts</li>
<li>Electrophoretic separation of digested parts</li>
<li>Isolation of DNA samples from gel</li>
<li>Isolation of DNA samples from gel</li>
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</html>
</html>
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===Cloning the p53 coding sequence===
 
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'''Marcin'''
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__NOEDITSECTION__
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====Comment:====
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<html>
-
Due to force problem with obtaining PCR product it was necessary to transform bacteria with plasmid containing p53 sequence and to perform all procedures once more time
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<h3><div style="text-align: center;">Cloning of p53 coding sequence</div></h3>
 +
<h4>Marcin</h4>
 +
<br/>
 +
<p><b>Comment:</p></b>
 +
<p>Due to problem with low quality of PCR product it was necessary to transform bacteria with plasmid containing p53 sequence and to perform all procedures once more time.</p>
 +
<br/>
 +
<p>Tasks:</p>
 +
<ul>
 +
<li>Transformation of chemocompetent strain of E. coli by plasmid containing p53 sequence</li>
 +
</ul>
 +
<p>Procedure:</p>
 +
<ul>
 +
<li>The protocol of transformation has not been changed, except of giving 1 &micro;l of plasmid solution to the bacteria. If you want to see detailed procedure go <a href="https://2009.igem.org/Team:Warsaw/Calendar-Main/7_July_2009">here</a>
 +
</li>
 +
<li>Bacteria was plated on the medium containing kanamycin</li></ul>
 +
 
 +
</html>
 +
 
 +
 
 +
===Construction of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012 K177012] operon1_part2===
 +
 
 +
'''Ania'''
Tasks:
Tasks:
-
*Transformation of chemocompetent strain of E. coli by plasmid containing p53 sequence
+
*Competent cells were transformed with LacI C0012 taken out of the distribution 2009 Kit Plate 1 well 20.
-
Procedure:
 
-
*The protocol of transformation has not been changed, except of giving 1 &micro;l of plasmid solution to the bacteria. If you want to see detailed procedure go [https://2009.igem.org/Team:Warsaw/Calendar-Main/7_July_2009 here]
+
===<div style="text-align: center;">Isolation of BioBricks from 2008 and 2009 Kit Plates</div>===
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*Bacteria was plated on the medium containing kanamycin
+
 +
'''Monika'''
 +
Tasks:
 +
*Isolate plasmids containing the biobricks - continuation
 +
 +
# GFP coding device switched on by IPTG - [http://partsregistry.org/Part:BBa_I763004 <span style="color: black;">BBa_I763004</span>]  from 2008 Kit Plate 1017 well G5
 +
# promoter lambda (cI regulated) with RFP reporter [http://partsregistry.org/Part:BBa_I763007 <span style="color: black;">BBa_I763007</span>]  from 2009 Kit Plate 1 well 15J
 +
# GFP generator - [http://partsregistry.org/Part:BBa_E0840 <span style="color: black;">BBa_E0840</span>] from 2009 Kit Plate 1 well 12O
 +
# GFP generator - [http://partsregistry.org/Part:BBa_J07037 <span style="color: black;">BBa_J07037</span>] from 2009 Kit Plate 1 well 12O
 +
 +
<br>
 +
 +
Results from 9 July 2009
 +
* all transformations exept GFP coding device switched on by IPTG (BBa_I763004) were successful
 +
 +
Methods:
 +
*Planting on LB medium supplemented with apropriate antibiotic, 6h incubation in 37&deg;C
 +
*Planting on LB-agar medium supplemented with apropriate antibiotic
 +
*Alkaline lysis (prodedure described [http://www.aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf <span style="color: blue;"> here</span>])
 +
 +
<br>
 +
 +
Results
 +
*Concentration of DNA sample was measured using NanoDrop ND-1000
 +
<br>
 +
{| class="wikitable" style="text-align:center; width:400px; height:90px; border-collapse: collapse; border: 1px dashed blue; margin: 1em auto 1em auto" border="1"
 +
|-
 +
!  DNA sample
 +
!  DNA concentration in ng/&micro;l
 +
|-
 +
|  [http://partsregistry.org/Part:BBa_I763007 <span style="color: black;">BBa_I763007</span>]
 +
|  12.63 and 17.95
 +
|-
 +
|  [http://partsregistry.org/Part:BBa_E0840 <span style="color: black;">BBa_E0840</span>]
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|  34.91 and 25.66
 +
|-
 +
|  [http://partsregistry.org/Part:BBa_J07037 <span style="color: black;">BBa_J07037</span>]
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|  12.52 and 48.68
 +
|}
 +
<br>
 +
Comment
 +
*Isolations where DNA concentration is under 30ng/&micro;l must be repeated
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{{WarNotebookEnd}}
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Latest revision as of 21:17, 19 September 2009


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Team meeting

  1. presentations of work did by both groups during last week (given by Ania and Kuba)
  2. presentation of methods used by teams starting in previous editions of iGEM to kill bacteria :) (Kamil)
  3. presentation of different methods of targeting drugs to cancer cells (Marcin)

We've been also talking about some organization issues and we've decided to move our meetings - now they will take place every Thursady at 17:00, one week at the Faculty of Biology Building, another week in the room E of the lab on Pawinskiego Street.


Creating devices to test promoters in E. coli strains (devices [http://partsregistry.org/Part:BBa_K177024 BBa_K177024] and [http://partsregistry.org/Part:BBa_K177025 BBa_K177025])

Franek


Tasks:

  • Alkaline lysis of the plasmid containing [http://partsregistry.org/Part:BBa_B0024 BBa_B0024 terminator]


Methods:

  • [http://aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf PlasmidMini set by A&A Biotechnology] was used. 2 test tubes with 5ml LB and ampicillin were first inoculated with colonies containing [http://partsregistry.org/Part:BBa_B0024 BBa_B0024] brick on [http://partsregistry.org/Part:pSB1A2 pSB1A2] plasmid. The cultures were incubated for 6h at 37°C. Alkaline lysis was performed on both cultures. The pellet from 5 ml of bacteria was used.


Results:

  • Concentration of DNA sample was measured using NanoDrop ND-1000


DNA sample DNA concentration in ng/µl
[http://partsregistry.org/Part:BBa_B0024 BBa_B0024] 33.82



Notes:

  • Plates with [http://partsregistry.org/Part:BBa_I0500 BBa_I0500] transformants were empty once again. This result, together with note in [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_I0500 part description] suggest that this part is damaged

Making of RBS-cI part

Jarek


Task:

  • Digestion of parts BBa_C0051 and BBa_B0032
  • Electrophoretic separation of digested parts
  • Isolation of DNA samples from gel
  • Ligation of part C0051 to the vector with B0032

Methods:

  • DNA samples were digesteg with 1 µl of PstI/BcuI (B0032) or PstI/XbaI (C0051) in 1xTango buffer for 3 hours.
  • After digestion they were separated on 0,8% agarose gel.
  • Isolation of sample from gel was performed with the A&A "Gel-out" kit.
  • For ligation 4µl of ligase buffer and 2 µl of ligase were used.

Results:



Cloning of p53 coding sequence

Marcin


Comment:

Due to problem with low quality of PCR product it was necessary to transform bacteria with plasmid containing p53 sequence and to perform all procedures once more time.


Tasks:

  • Transformation of chemocompetent strain of E. coli by plasmid containing p53 sequence

Procedure:

  • The protocol of transformation has not been changed, except of giving 1 µl of plasmid solution to the bacteria. If you want to see detailed procedure go here
  • Bacteria was plated on the medium containing kanamycin


Construction of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012 K177012] operon1_part2

Ania

Tasks:

  • Competent cells were transformed with LacI C0012 taken out of the distribution 2009 Kit Plate 1 well 20.


Isolation of BioBricks from 2008 and 2009 Kit Plates

Monika

Tasks:

  • Isolate plasmids containing the biobricks - continuation
  1. GFP coding device switched on by IPTG - [http://partsregistry.org/Part:BBa_I763004 BBa_I763004] from 2008 Kit Plate 1017 well G5
  2. promoter lambda (cI regulated) with RFP reporter [http://partsregistry.org/Part:BBa_I763007 BBa_I763007] from 2009 Kit Plate 1 well 15J
  3. GFP generator - [http://partsregistry.org/Part:BBa_E0840 BBa_E0840] from 2009 Kit Plate 1 well 12O
  4. GFP generator - [http://partsregistry.org/Part:BBa_J07037 BBa_J07037] from 2009 Kit Plate 1 well 12O


Results from 9 July 2009

  • all transformations exept GFP coding device switched on by IPTG (BBa_I763004) were successful

Methods:

  • Planting on LB medium supplemented with apropriate antibiotic, 6h incubation in 37°C
  • Planting on LB-agar medium supplemented with apropriate antibiotic
  • Alkaline lysis (prodedure described [http://www.aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf here])


Results

  • Concentration of DNA sample was measured using NanoDrop ND-1000


DNA sample DNA concentration in ng/µl
[http://partsregistry.org/Part:BBa_I763007 BBa_I763007] 12.63 and 17.95
[http://partsregistry.org/Part:BBa_E0840 BBa_E0840] 34.91 and 25.66
[http://partsregistry.org/Part:BBa_J07037 BBa_J07037] 12.52 and 48.68


Comment

  • Isolations where DNA concentration is under 30ng/µl must be repeated

April
MTWTFSS
    [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_April_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_April_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_April_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_April_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_April_2009 5]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/6_April_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_April_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_April_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_April_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_April_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_April_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_April_2009 12]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/13_April_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_April_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_April_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_April_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_April_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_April_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_April_2009 19]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/20_April_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_April_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_April_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_April_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_April_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_April_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_April_2009 26]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/27_April_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_April_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_April_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_April_2009 30]
May
MTWTFSS
        [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_May_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_May_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_May_2009 3]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/4_May_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_May_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_May_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_May_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_May_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_May_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_May_2009 10]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/11_May_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_May_2009 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_May_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_May_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_May_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_May_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_May_2009 17]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/18_May_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_May_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_May_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_May_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_May_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_May_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_May_2009 24]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/25_May_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_May_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_May_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_May_2009 28] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/2_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/7_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/13_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/14_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/15_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_June_2009 16] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/17_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_June_2009 18] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/19_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/20_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_June_2009 23] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_June_2009 26] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/27_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_June_2009 28]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/29_June_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_July_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_July_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_July_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_July_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_July_2009 5]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/6_July_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_July_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_July_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_July_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_July_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_July_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_July_2009 12]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/13_July_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_July_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_July_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_July_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_July_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_July_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_July_2009 19]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/20_July_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_July_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_July_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_July_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_July_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_July_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_July_2009 26]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/27_July_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_July_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_July_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_July_2009 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_July_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_August_2009&preload=Team:Warsaw/NotebookPreload&action=edit 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_August_2009 2]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/3_August_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_August_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_August_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_August_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_August_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_August_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_August_2009 9]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/10_August_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_August_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_August_2009 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_August_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_August_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_August_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_August_2009 16]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/17_August_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_August_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_August_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_August_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_August_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_August_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_August_2009 23]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/24_August_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_August_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_August_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_August_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_August_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_August_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_August_2009 30]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_September_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_September_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_September_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_September_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_September_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_September_2009 6]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/7_September_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_September_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_September_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_September_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_September_2009 11] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_September_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_September_2009 13]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/14_September_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_September_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_September_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_September_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_September_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_September_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_September_2009 20]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/21_September_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_September_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_September_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_September_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_September_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_September_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_September_2009 27]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/28_September_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_September_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_October_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 4]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/7_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 11]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_October_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_October_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_October_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_October_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_October_2009 17] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/18_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 18]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/19_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/20_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/23_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/26_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/27_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/28_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]
Retrieved from "http://2009.igem.org/Team:Warsaw/Calendar-Main/10_July_2009"